| Objective:Cerebrovascular disease (cerebrovascular disease, CVD) has becometoday’s crimes against humanity, especially the common diseases of the elderlyharm and disease, with high morbidity, high mortality and high morbiditycharacteristics, not only bring patientspain, but also a heavy burden to the societyand the family. The purpose of this study is to investigate the serum HIF-1alphaexpression of Caspase-3with time, and hope to provide new ideas for effectiveclinical treatment and intervention of acute cerebral infarction with acutecerebral infarction lesion volume size, nerve function defect degree and methods.Methods:1.Case Source: All patients from the Sichuan Provincial People’s Hospital,Department of Neurology admitted with acute cerebral infarction patients andhealthy persons（October2012to February2013）.2.Groups: the establishment of case and control groups. Case group: onsetwithin12hours, are in line with the Fourth National Stroke Conference cerebralinfarction revised diagnostic criteria, confirmed by CT or MRI lesions werelocated in the internal carotid artery in patients with acute cerebral infarction30cases,18males and12females patients, aged40-80years, mean age62.20±9.05years old; control group:30healthy subjects collected in the same period asthe control group,16males and14females, aged39-80years, an average of60.98±8.77years old. The two groups were comparable in age, sex, bloodpressure, cholesterol and other basic information constitute (p>0.05).3.Specimens were taken and testing: the case group were the first12h after theonset, ld,3d,5d,7d,10d take antecubital venous blood3ml, take the upperserum at-80°C refrigerator storage spare; control group to take the antecubitalvenous blood3ml serum supernatant at-80°C refrigerator storage spare. Thespecimens were detected by double antibody sandwich enzyme-linkedimmunosorbent assay (ELISA) serum HIF-1alpha, Caspase-3concentration. 4.Nervous system rating: recorded in patients with acute cerebral infarctionneurological deficit score (NIHSS National Institutes of Health Stroke Scale) atdifferent time points (after the onset of the first12h,3d,7d,10d).5.Infarct volume: all patients with cerebral infarction group, the incidence3dcranial MRI results to calculate the volume of cerebral infarction lesions;reference Pullicino, infarct volume=a×b×c×slice thickness×pi/6(where ais the maximum diameter of the infarct, b is the diameter perpendicular to thediameter, c MRI scan positive number of layers, pi pi); divided into3groupsaccording to the volume of cerebral infarction lesions size: small infarction (≤4.0cm3); infarction (4.1-10.0cm3); infarction group (≥10.0cm3).6.Data processing: All data were statistically analyzed with SPSS19.0software package to complete the establishment of a significance level alpha=0.05, p <0.05was considered statistically significant between the two groupssamples t-test was used to compare multiple samples were compared usingone-wayANOVA, correlation analysis using Spearman rank correlation method.Results:1.HIF-1:1) the incidence of acute cerebral infarction serum HIF-1αexpression detected after12h, a significant difference compared with the controlgroup, after the onset of the first day reached a peak, followed by concentrationgradually decreased over time; after the onset of the first day,3d,5d,7d,10dserum HIF-1α concentration and normal control group were statisticallysignificant differences;2) cerebral infarction volume group: compared to thevolume of the cerebral infarction group and the normal control group comparedat each time point (12hours after the onset,1d,3d,5d,7d,10d) are significantlydifferent (p<0.05); small infarction and infarction group significant difference(p>0.05); small infarct group with large infarction group at each phase point ofcomparison were significant the difference (p<0.05); in the infarction group withlarge infarction group at each phase point of comparison were significant thedifference (p<0.05). 2.Caspase-3:1) in patients with acute cerebral infarction incidence12hdetected serum Caspase-3expression, a significant difference compared with thecontrol group, after the onset of1d reached a peak concentration thereaftergradually decline over time; after the onset of the first day,3d,5d,7d serumconcentration of Caspase-3and the normal control group, p<0.05, significantdifference;10d both compared p>0.05).2)cerebral infarction volume group:small infarction and infarction serum concentration of Caspase-3, the two groupsafter the onset of the first12h,1d,3d,5d,7d,10d comparison, p<0.05significantdifference; small infarct group with large infarction group serum concentrationof Caspase-3comparison, after the onset of each time point (after the onset of12h,1d,3d,5d,7d,10d) have significant differences; in the infarction group andThe infarction group serum concentration of Caspase-3was significantdifference in the expression of the respective time point-like.3.Serum HIF-1α and Caspase-3expression: cerebral infarction in30patientsserum HIF-1α and Caspase-3concentration was Spearman correlation analysis,the result is serum HIF-1α and Caspase-3concentration in the12hours after theonset of1d,3d,5d,7d,10d, six time points expression was no significantcorrelation (p>0.05).4.Serum HIF-1α expression of Caspase-3concentration and nerve functiondeficit score correlation:1) serum HIF-1α concentration and NIHSS after theonset of cerebral infarction patients,12hour,3d,7d,10d carried NIHSS score,with the corresponding timepoint serum HIF-1α concentration Spearmancorrelation analysis results p>0.05in the four time points was no significantcorrelation between the two Caspase-3.2) Concentration of serum Caspase-3and NIHSS, after the onset of cerebral infarction patients,12hour,3d,7d,10dcarried NIHSS score, with the corresponding time points serum concentration ofCaspase-3Spearman correlation analysis are displayed in the four time points nosignificant correlation (p>0.05). Conclusion:1.Dynamic changes of serum levels of HIF-1alpha expression, indicating thatthe factors involved in the occurrence and development of acute cerebralinfarction, cerebral infarction after a series of hypoxia response play animportant role.2.Serum levels Caspase-3expression of the dynamic changes that the factorsinvolved in acute cerebral infarction pathophysiology of acute cerebral infarctionand apoptosis.3.Serum levels of HIF-1alpha expression of Caspase-3’s may be related to theinfarct lesion volume.4.Acute cerebral infarction patients with serum expression of HIF-1α,Caspase-3expression without significantly related.5.The serum levels of HIF-1α concentration and NIHSS score no significantcorrelation, serum expression of Caspase-3concentration with NIHSS score wasno significant correlation. |
PDF Full Text Request