A Study Of Activate LXR To Protect The Ischemia-reperfusion Injury Of Transplanted Livers In Rats

Objective To investigate the protective effects of liver X receptoragonist injecting pretreatment in ischemia-reperfution injury of rat livergrafts.Methods Fouty-eight male Sprague Dawley rats, weighted200～250g, were used as donors(n=24) and recipients(n=24) of liver transplantation.They were randomly divided into LXR agonist GW3965group(n=12) withdonor liver injected with GW3965(0.3mg/kg) though the caudal vein thirtyminutes before operative versus the control group(n=12), with liversinjected the same amount of saline.3h,6h,24h after the transplantation,the level of alanine aminotransferase(ALT) in each group were assessedwith automatic biochemistry analyzer; The protein expressions of IRAK-4and IRF3were detected by western-blot; the activity of NF-κB wereobserved by EMSA; The densities of TNF alpha in serum were evaluatedby enzyme-linked immunosorbent Assay(ELISA); The morphologicalobservation of hepatic tissue under light microscope.Results At each phase, serum levels of ALT and the densities of supematant TNF-α in GW3965group was obviously compared with thosein control group (P<0.05). The protein expressions of IRAK-4and IRF3and the activity of NF-κB in GW3965were obviously lower than these incontrol group at each time point (P<0.05). At each phase, hepatic injury inGW3965group was significantly lighter than it in control group underlightmicroscope.Conclusion: Activating LXR can significantly palliate theischemia-reperfusion injury after liver transplantation by down-regulatingthe levels of IRAK-4and IRF3protein expression and suppressing theactivity of NF-κB, and partially release the inflammation.