Effect Of Overexpression Beclin1on Esophageal Cancer (eca109) Cells In Vivo

Objective：To investigate the effect of overexpress Beclin1on esophagealcancer.Method：42cases of esophageal squamous cell carcinoma tissue and20cases of normal esophageal tissue was investigated byimmunohistochemistry SP method.Beclin1recombinant plasmidpIRES2-ZsGreen1-hBeclin1was constructed and transfected into Eca109cells. Autophagy was observed under electron microscopy. The expressionof Beclin1at mRNA level was detected by the reversetranscription-polymerase chain reaction (RT-PCR), and western blot wasused to examine the protein expression of Beclin1, Bcl-2and P53.Moreover, in vivo, the proliferation of the cancer cells was evaluated inxenotransplant nude mice model.Result： The results showed that reduced Beclin1expression was obsemedin esophageal cancer than in normal tissuls(P<0.05). We successfullyconstructed Beclin1recombinant plasmid and transfected into Eca109cells, autophagosomes were widely formation. The results of RT-PCR andwestern blot showed that the expression of Beclin1was markedly higher intransfected group ECA109cells at both mRNA and protein levels than thatof control group and untransfected group. In addition, the proteinexpression of Bcl-2was down-regulated but P53up-regulated aftertransfected. In vivo, the average weight and volume of tumor in Beclin1transfection group xenografts mice were significantly lower than those inthe control group(P<0.05).Conclusion： The result suggested that Beclin1gene have a goodinhibition for esophageal cancer, and this lay the foundation for targetedtherapy for esophageal cancer.