| Background:Total Joint Replacement has been widely used in clinical; it reduces the patient’s suffering, which greatly improved the patient’s quality of life. Aseptic loosening has been the most common postoperative complications, which directly shorten the life of the prosthesis, are the main reason of postoperative renovation. Recent studies show that the release of wear particles from the interface between implant component and surrounding bone cause increased release of inflammatory mediators, which in turn promotes osteoclastogenesis and bone resorption, and subsequently leads to implant loosening. We use a gauze drying bone and surrounding tissue bleeding in surgery. Gauze fibers can be left in the implant, it as the non-medical transplantation material which left in the human body will cause a foreign body reaction to the formation of chronic granulomatous.Polyethylene wear particles often lead to aseptic loosening. The experiment focuses on inflammatory response induced by polyethylene wear particles and gauze fibers respectively whether there are differences, to speculate whether gauze fibers can induce osteolysis. Objective:To investigate whether gauze fibers can induce osteolysis in mouse air pouch model. To compare osteolysis effects induced by gauze fibers and polyethylene wear particles respectively. To discuss the association between gauze fibers and aseptic loosening.Methods:Air was injected into the mouse back to form an air pouch, and then implant the skull bone from another homologous mouse into the air pouch to obtain an air pouch model. Mice were divided into three groups:gauze fibers group (gauze fibers and physiological saline were injected into the air pouches); particle group (Polyethylene wear particles and physiological saline were injected into the air pouches); blank group (physiological saline was injected into the air pouches).17days later, all the mice are sacrificed, pouch walls and implanted calvaria were collected. The inflammatory reaction and the number of exuded inflammatory cells of the pouch walls were detected through HE staining. The differentiation and maturation of osteoclast were observed through tartrate-resistant acidic phosphatase (TRAP) staining. Immunohistochemical staining was used to examine the expressions levels of inflammatory cytokines IL-1, IL-6and TNF-a. The expressions of TNF-a mRNA and protein was detected by Real-Time PCR and ELISA.Results:(1) HE staining results showed that the number of exuded inflammatory cells of the pouch walls in the gauze fibers group and the particle group were significantly higher than that in the blank group (p<0.01), and there was no difference between the gauze fibers group and the particle group(P>0.05).(2) The percentage of tartrate-resistant acidic phosphatase (TRAP) staining positive area to calvaria area in the gauze fibers group and the particle group were significantly higher than that in the blank group (p<0.01), and there was no difference between the gauze fibers group and the particle group (P>0.05).(3) The expressions levels of inflammatory cytokines IL-1and IL-6examined by Immunohistochemical staining in the gauze fibers group and the particle group were significantly more intense than that in the blank group (p<0.01),and there was no difference between the gauze fibers group and the particle group (P>0.05). The levels of TNF-a in the gauze fibers group and the particle group were significantly more intense than that in the blank group (p<0.01), and the gauze fibers group was more intense than the particle group (p<0.05).(4) Real-Time PCR and ELISA results showed that the expressions of TNF-a mRNA and protein in the gauze fibers group and the particle group were significantly higher than that in the blank group (p<0.01), and the gauze fibers group was higher than the particle group (p<0.05).Conclusion:Gauze fibers could cause the release of local inflammatory cytokines, which induce periprosthetic aseptic inflammation. |
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