Study On Separation Performance Of L-Arabinose In Corn Husk Hydrolyzate By Zymotechnics

L-arabinose is one of the new functional monosaccharide, which plays a role in controling blood-sugar content, inhibiting fat formation and proliferating of Bifidobacterium, etc. Extracting highly purified L-arabinose quickly and efficiently has become a hot research. Hemicellulose acidolysis has been widely used to produce L-arabinose in industry currently. The product is a kind of mixture contained several monosaccharides. The current method of using resin or thin layer chromatography to extract L-arabinose is complicated and inefficient. This text studied on separation of L-arabinose by microorganism zymotechnics. Corn husk hydrolyzate contained three primary monosaccharides, namely glucose, xylose and L-arabinose. This experiment investigated the circumstances of the three monosaccharides utilized by different microorganism.First of all, raw material and the method of preliminary extraction were screened. Sulfuric acid was used to hydrolyze corn husk to prepare hydrolyzate. HPLC was used to determination the content of main monosaccharides in corn husk hydrolyzate. The results showed glucose, xylose and L-arabinose contained6.11%,18.82%and11.34%, respectively. And according to this, a analog culture medium was prepared.Afterwards, common five strains yeast, seven strains mould, seven strains bacteria, four strains lactobacillus and Mgbl, Mgb2which were separated and screened from environment were fermented in analog culture medium. Medium was detected by HPLC during fermentation. The results indicated that glucose could be consumed completely by all bacterial strains within1-2days. Bacteria and lactobacillus were not suite for separation of xylose and L-arabinose. Torulopsis spp., Trichoderma viricle, Botrytis cinerea, Mgbl and Mgb2have good selectivity to separating xylose and L-arabinose. Most xylose can be consumed by these five strains after3days fermentation. Residual rate were only5.8%,2%,5.2%,4.5%and3.9%respectively. While L-arabinose still remained substantially. Retention rate were83.4%,70%,76.8%,88.6%and77.7%respectively.Corn husk hydrolyzate was fermented by Torulopsi spp., Trichoderma viride、Botrytis cinerea, Mgb1and Mgb2which were screened by analog culture medium and detected by IIPLC during fermentation. The results showed that residual rate of xylose was less than5%after3days fermentation by Torulopsi spp.,4days fermentation by other4strains, while the retention rate of L-arabinose was more than75%. This separation method is simple and efficient. Tondopsis spp., Trichoderma viride and Botrytis cinerea are common and accessible. It provided reference for separation of xylose and L-arabinose by microorganism zymotechnics some extent.