The Effect Of Feeding Different Density Of D-fructose On Rats’physiological Function

In the past20years, the usage of fructose as a sweetener increased from20%to30%in food industry. With the rapid growth of production and consumption, people gradually realized the unique advantages of fructose and started to pay attention to its nutritional characteristics and food safety. Recent years, there are many studies about nutrition, food safety and metabolic pathway related to fructose. With feed containing10%glucose and basic feed as control,28-day-old rats were fed feed that containing10%,20%,30%fructose, to study the effect of different concentrations of fructose diet on health of rats. Findings were as follows:1. The change of food-intake and body weight of rats were recorded every four days, and it was found that different concentrations of fructose and low concentrations of glucose diet did not cause weight gain and obesity, and fructose concentration was negatively correlated with body weight, rats that fed high concentration of fructose were underweight.2. The content of LDL, HDL, CHO, TG, LP, INS in rats’serum were detected every14days, and it was found that fructose and glucose had a big impact on the content of LDL, HDL, CHO and TG in rats’serum, especially during juvenile stage. Fructose diet in different concentrations had different effects on blood parameters. The content of LDL, HDL, CHO and TG increased, while HDL/LDL did not change significantly in rats which were fed by fructose in low concentrations. In rats fed by fructose in high concentration, the content of LDL and HDL increased, while the HDL/LDL decreased at the same time, the content of CHO increased more than others and the content of TG did not change significantly. LP resistance and INS increase were led by fructose diet in either high or low concentration, and effect of low concentration was more obvious. Glucose in low concentration had no significant effect on LP and INS.3. In the56th day, liver samples were collected and weighed. The content of alkaline phosphatase (ALP), stearoyl coenzyme A desaturase (SCD), acetyl coenzyme A hydroxylase (ACC), pyruvate kinase (PK) and fatty acid synthase (FAS) in livers were detected. It was found that lipid synthesis in livers was promoted by fructose feed, either in high or low concentrations. The pathological change in liver was found in rats which were fed by high concentrations fructose diet.It was showed in the experiment that rats’physiological metabolism, growth and development were affected differently by fructose diet of different concentration.