| Objective: To investigate the effect of repeated ketamine administration on p-cofilinand cofilin in rat hippocampus and their possible mechanism be involved in ketamineresulted in the cognitive dysfunction.Methods: Forty2-month-old male Sprague-Dawley rats were randomly divided intotwo groups: ketamine group (K group) and normal saline control group (C group).Rats in K group had been intraperitoneally injected with50mg/kg ketamine, and thosein C group had been administrated with1ml/kg saline for one week. After twodays,Morris water maze test (MWM)was adopted to explore the latency in placenavigation(PNT) and spatial probe (SPT) ability. Through the morris water maze test,the cognitive deficit model group (M group) was screened from the ketamine group.The expression of p-cofilin and cofilin in hippocampus of the cognitive deficitgroup and saline control group rats were analysed by Western blot.Results:(1)In Morris water maze test, the latency in place navigation of K group wassignificantly longer than that of C group (P<0.05), and the spatial probe abilitysignificantly decreased (P<0.05).(2)In western blot test, it was revealed that theexpression of p-cofilin in M group was significantly lower than that in Cgroup(P<0.05),while there was no significant difference in the of expression of cofilinbetween these two groups (P>0.05).Conclusion:(1) Repeated administration of ketamine can cause cognitive dysfunction in rat;(2)The expression of p-cofilin reduce in cognitive dysfunction ratshippocampus;and changes of cofilin activity might be involved in ketamine resulted inthe cognitive deficit mechanisms. |
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