Study On Quality Standard Of Rubi Fructus

Objective:1.To perfect raspberry herbs quality standars Raspberry included in Pharmacopoeiareproduces commonly used in traditional Chinese medicine,After severalPharmacopoeia Amendment, To the2010version of a pharmacopoeia qualitystandards only character identification, microscopic identification, Raspberry qualitystandards needs to be improved and perfected.2.Determine the best raspberry harvest period In addition, the raspberry best harvestresearch rare reports, In this study, the different origin, different harvest raspberries asthe research object, Research the dynamic changes of the chemical composition. Inorder to provide the basis for its determination of the optimum harvest date.Methods:1. Establish method for evaluating raspberry herbs1.1Establish TLC identification and the electrochemical fingerprint identificationmethod.1.2Compare different origin different harvest raspberry extract content.1.3Establish raspberry flavonoids content determination.1.4Establish method for the determination of organic acids composition.1.5Comparison of different origin and different harvest raspberries Mn, Zn andharmful elements content2. Intended raspberry draft of quality standards.3.Analysis harvest experimental data,Determine the best raspberry harvest period.Result:1.TLC conditions：ethanol as extraction solvent,Ethyl acetate-methanol–water（22：1：1）As the agent, To linden glycosides as control, The0.5%ethanol solutionof aluminum trichloride as chromogenic reagent,UV light (365nm) view spots,Tostudy raspberries in the TLC conditions, And to compare with Containing the lindentree glycosides herbs misty flower Effects of PDB, Agrimony. The results showed raspberries and other Chinese herbal medicines containing linden tree glycosides andraspberry adulterants significant difference on thin layer chromatography. Thismethod can identify the raspberries with raspberry adulterants and with linden treeglycosides Chinese herbal medicines.The establishment of the electrochemical fingerprint: Selection of H2SO4-CH2(COOH)2-Ce (SO4)24H2O-KBrO3as oscillating chemical system,Raspberry asoscillation substrate, Establish the electrochemical fingerprint. And compareelectrochemical oscillation spectrum with Rosaceae herbs papaya, hawthorn, plum,the Jinyingzi. The results showed significant difference in the electrochemical map ofraspberries and other Rosaceae herbal. The method can identify raspberries and otherRosaceae fruits medicine.2.The2010version of "Chinese Pharmacopoeia" an Appendix extract as a reference,Selection of hot-dip method for the determination of raspberries in water, alcoholextract content, Provide the basis for the raspberry extract determination. The resultsshow raspberry water extracts in the range of15.44%-23.18%,Alcohol extractcontent of7.97%-17.07%. Different harvest raspberry fruit,higher levels of waterand alcohol extract content nearly ripe fruit and ripe fruit content.3.Establish UV spectrophotometry determin different origin, different harvestraspberries total flavonoids; Establish HPLC chromatography determin differentorigin, different harvest flavonoids linden tree glycosides, rutin, quercetincontent.Analysis of the measured results. The results show that the the different originraspberry total flavonoids, the flavonoids linden tree glycosides, rutin, quercetincontent there are significant differences. The different origin raspberry totalflavonoids With increase in fruit maturity,Increased and then decreased. The highestcontent in the fruit becomes green yellow.The linden tree glucoside、three kinds offlavonoids and total flavonoids change trend consistency, With increase in fruitmaturity,Increased and then decreased. Establish a potentiometric titration method forthe determination of total organic acid content of different origin and different harvest raspberries. Establish an HPLC method for the determination of organic acidsingredients citric acid, gallic acid. The results show that the the different originraspberry total organic acids, organic acids ingredients citric acid, gallic contentdifferences, Different origin raspberry soluble organic acids content increased with thedegree of maturity of the fruit increased, The highest total organic acid content in thefruit color changes to red.4.The application of ICP-MS method to determine harmful elements and beneficialelements the content of raspberry. The results showed that the five kinds of harmfulelements were not excessive. Different harvest samples beneficial elements Mncontent of a regularity, With the increase of the degree of maturity,First increased andthen decreased.Zn content changes have no obvious ruleConclusion:The subject from the identification (TLC, electrochemical identification)、determination of extract、 determinationr aspects, Supplement and improve theraspberry herbs quality standards, Provide some basis for the establishment of thequalitative and quantitative analysis and evaluation of the raspberry.Raspberry harvest period, Pharmacopoeia provision early summer fruit from green toyellow to green when harvested. From the measurement results, Raspberry totalflavonoids reached the highest levels in the mid to late fruit from green to green-yellow, The harvest period consistent with the provisions of the Pharmacopoeia,Water-soluble organic acid content increased in the extension of Raspberry. Thehighest content of red fruit in early June.