| Background Gastrointestinal dysfunction in acute pancreatitis is recongnized as one of the common clinical symptoms, which can cause Intra-abdominal hypertension recongnized as prognostic marker for severity of severe acute pancreatitis. Chinese medicine believes that the "lung and large intestine are interior exteriorly related". Previous studies indicate that acupuncture can improve gastrointestinal motility and beneficial in the treatment of acute injury of lungs acute pancreatitis. The purpose of this experiment was to study electroacupuncuture for ameliorating acute lung injury through regulating gastrointestinal motility in rats with acute pancreatitis.Object To test hypothesis"lung and large intestine are interior exteriorly related"through investing into the effect of electroacupuncture on serum vasoactive intestinal peptide (VIP) and Cholecystokinin (CCK) in acute pancreatitis rats.Method Male SD rats were randomly divided into electroacupuncute (EA), model and normal groups. Acute pancreatitis model of EA group and model group is established by injection of intraperitioneally with20%L-arginine (1g/kg) every hour within3hours. EA (2Hz/100Hz,2mA) was applied to bilateral ST25and ST-36for30min every12h after modeling and at the end of the experiment. After72h the rats were killed, aterial blood, pancreatic, lung and intestinal tissues were collected for bimarkers and histopathology. Interstinal propulsion rate was also measured.Results EA treatment reduced serum VIP and lung MPO level and decrease the pathologic score of pancreatic, lung and intestinal tissues compared with model group (P<0.05). EA treatment improved the intestinal propulsion rate and increased serum CCK level in AP rats compared with model group (P<0.05); serum CCK in normal group is higher than EA and model groups (P<0.05).Contusion EA treatment can improve gastrointestinal motility through inhibiting VIP and promoting CCK expression to ameliorating acute lung injury in AP rats. |
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