Study About Effect Of Rats Pulmonary Disease On Large Intestine And Its Pathological Mechanism Based On TGFβ1/Smad3and EGF Signaling Pathway

Objective:Establish pulmonary disease (chronic bronchitis) animal model, then select three different time points and observe the expression of TGF-β1、TGF-βRI、 EGF and ErbB3between lung and large intestine, thus exploring the pathological change mechanism and material basis of" pulmonary disease affects large intestine’Methods:Select60male SD rats and randomly divided into blank group (30) and model group (30) by weight. Put the blank group rats into smoke-free environment, meanwhile, put the model group rats into the smoke environment in order to establish pulmonary disease (chronic bronchitis) animal model, totally smoked70days. Observe pathological morphology change of lung, stomach, duodenum, jejunum, ileum, colon, and rectum. Test TGF-β1、TGF-βR1、Smad3、EGF、ErbB3expressions in lung and colon tissue of rats.Result:1. Pathological morphologyLight:The bronchial epithelial cells of lung tissues were wide degeneration, necrosis and different degree inflammatory cells in model group rats on the20th,50th and70th days. There are respectively20%、70%、90%of the colon tissues appeared local congestion edema and the epithelial cells were wide degeneration and necrosis in model group rats on the20th,50th and70th days. The colon tissues of some rats appeared mucosal surfaces were incomplete and the glands arrangement weren’t regular. Mucosa and sub mucosal cells were wide inflammatory. The duodenum, the jejunum, ileum, rectum, stomach were all normal.2. TGF-β1、TGF-βR1、Smad3、EGF、ErbB3expressionCompared with blank control group, the expression of TGF-β1、TGF-βRI、 Smad3、EGF、ErbB3increased significantly in lung tissue and the expression of TGF-β1、TGF-βR1、Smad3、EGF、ErbB3increased significantly in colon tissue in model group rats on the20th,50th and70th day (P<0.05or P<0.01). Compared with the20th day, the expression of TGF-βR1、Smad3increased in colon tissue in model group rats on the50th days (P<0.05or P<0.01), the expression of TGF-β1、TGF-βR I、smad3、ErbB3decreased in lung tissue andTGF-βRI、smad3、ErbB3in colon tissue of model group rats on the70th days (P<0.05or P<0.01). Compared with the50th day, the expression of TGF-β1、EGF、ErbB3decreased in lung tissue and the expression of smad3decreased in colon tissue in model group rats on the70th days (P<0.05or P<0.01)Conclusion:1. Pulmonary disease rats can appear pathological changes of the large intestine. It means that pulmonary disease probably affected large intestine; After pathologic observation of gastrointestinal obably affesegments, intestinal damage was mainly embodied in the colon.It was the colon that pulmonary disease probably affected.2.1t was a sustained chronic process of pulmonary disease involving large intestine.It took about50d for model rats’chronic bronchitis causing large intestine injury.3.TGF-β1may be one of the material basis about pulmonary disease affected large intestine.TGF-β1/Smad3signaling pathway may participate in the pathologic transmission.4.EGF may be one of the material basis about pulmonary disease affected large intestine.