The Research On The Effect Of Ganduqing Buccal Tables To The Regulation Of Immunity In Immunosuppressed Mice

ObjectiveIn recent years, With the period of the increasing in atmospheric pollution and aged tendency of population, respiratory diseases caused more and more attention,Chinese medicine can benefit the human body, improve human immunity, enhance the body fighting the infection of pathogenic microorganisms, and play an important role in the prevention and treatment of respiratory diseases.This experiment of animal experimental illustrates the new drug Ganduqing buccal tables can enhance immunity, expounds its popularization in clinical, the feasibility of the prevention and treatment of influenza virus infection.MethodsThe study uses randomized,controlled,double-blind experimental design methods.Purchase60ordinary level Kunming mice.Equal number of male and female. all mice were randomly divided into6groups,except the normal control group.In order to cause immune suppression model, injecting cyclophosphamide80ml/kg, once a day for three consecutive days,GDQ in high dose,middle dose and low dose,yupingfengsan(a TCM decoction)Gavage once daily for seven consecutive days,Using flow cytometry to observe NKT cells, Th17cells percentage changes in the blood of mice.By enzyme-linked immunosorbent assay(ELISA) to research cell factor β-defensin-2, IFN-y,IL-2,IL-4content changes in the thymus tissue homogenate.Results1comparing with cyclophosphamide model group,after gavage ganduqing(GDQ) to mice for7days,NKT cells were significantly increased in the blood of mice and showed a dose-effect relationship,The effect of the high-dose group is most obvious. percentage of Th17cells in CD4+T tended to increase in blood, but no statistically significant (P>0.05). 2gavage ganduqing(GDQ) for7days, then take the thymus tissue make into thymus tissue homogenate.compared with cyclophosphamide model group,The level of βD-2、IFN-γ、IL-2significantly increased,and IL-4obviously reduced.(P<0.05).ConclusionIn the state of immunity is suppressed.gavage ganduqing(GDQ) to the experimental mice could improve immune-related indicators.Reduce immunosuppressive factor expression.This experiment prove that it has a strong role in strengthening the immunity.The results of the experiment laid a solid theoretical foundation for ganduqing(GDQ) buccal tables further promoted to the pharmaceuticals market and Applied to the clinical treatment.