Investigation On Quality Control Method Research And Sedative Mechanism Of Yixinningshen Pills

ObjectiveInvestigation and improve the quality standard of Yixinningshen pills. Studyof Yixinningshen pills on the effects of sleep deprivation on hippocampalneurons in rats, and to explore its mechanism of action.MethodsThe TLC method was used to identify the Total Ginsenoside of Ginsengstems and leaves, Schisandra chinensis and Ganoderma lucidum (Fr.) Karst inthe in the preparation. The RP-HPLC method is used for schisandrin andγ-Schisandrin in Yixinningshen pills. The flow rate was1.0mL min-1and thecolumn temperature was40℃. Using multiple platform method to make rat SD(sleep deprivation) model. Using HE staining, Nissl staining, and observing themorphology of neural cells by optical microscope, and using the S-P method forimmunohistochemical staining. Using the Image Pro Plus to analysis, statistic ofthe data. To observe the structure of hippocampus CA3cell ultrastructralchange by electron microscope.ResultsThe TLC spots were fairly clear and the negative control showed nointerference. There was good linearity between the peak aeea and samplevolume within the ranges of0.5μg~20μg range.Regression equation were: Y=1.103×10~6X-1.997×10~,(r=1.0000);Y=2.636×10~6X+2.481×104,(r=0.9999). Schisandrin average content of RSD is1.6448%;theaverage content of schisandrin RSD2.2024%. Compared with the modelgroup, the expression of drug group5-HT significantly decreased, BDNFexpression increased significantly, and the difference of the positive expressionin normal group is not. Multiple platform method was used to make SD model forthe drug group and SD group. Electron microscopy was used to observe thenerve cell morphology in hippocampal CA3area. Under transmission electronmicroscope, SD can lead to neuronal cells in rat hippocampal CA3areashrinkage, hyperchromatic nuclei, mitochondria in cells swelling, deformation,mitochondrial cristae vague, disorder, endoplasmic reticulum expansion, lacuna.The nerve cells and intracellular structure damage in drug group is obvious lessthan SD group.ConclusionsThe TLC method can be used to identify the Yixinningshen pills, and that isdependable and practical. With high sensitivity, simple operation, accurateresults,the RP-HPLC method is suitable for the simultaneous determination ofschisandrin and γ-Schisandrin in Yixinningshen pills. We can see the protectiveeffect of SD rats caused by damage to the hippocampus, which may be theresult of nerve cells protection,5-HT reducing and BDNF increasing. PromptSD can cause ultrastructure pathological changes of nerve cells in rathippocampal CA3area. Yixinningshen pills has a significant therapeutic effect tothe damage baused by SD.