The Inhibitive Effect Of Freezing On Reperfusion-induced Oxidative Stress Injury In L02Hepatocytes

Primary hepatic carcinoma is one of the most popular diseases in mankind. China is acountry with high rate of viral hepatitis, so the number of viral hepatitis-induced primaryhepatic carcinoma is growing year by year. The high rate of recurrence and transfer afteroperation has great influence on the prognosis of the patients. It is important to find aneffective way to prevent the recurrence and transfer of the tumor. Extracorporeal liverresection and partial liver autotransplantation became a good choice for primary hepaticcarcinoma patiens since the year of1988. In our division, we have had38operations ofextracorporeal liver resection and partial liver autotransplantation. After long time ofclinical observation we found better liver functional rehabilitation in patients withoperations of extracorporeal liver resection and partial liver autotransplantation than inpatients with ordinary operations.One of the key points in the operations of extracorporeal liver resection and partialliver autotransplantation is freezing. Recently more and more researchers focused on theprotective effect of freezing. They found that freezing could inhibit reperfusion injury bydecreasing IL-8expression in heart and increasing Bcl-2/bax expression rate in smallintestine. In our present study, we aim to explore the mechanism by which freezing inhibitsreperfusion-induced oxidative strss injury. We established an reperfusion injury modelinduced by UW liquid, measured by the cell viability, cell apoptosis, the level of MDA,LDH, SOD and intracellular ROS, as well as cell apoptosis, respectively. And then theeffects of freezing on endothelial vitality, cell apoptosis, the level of MDA, LDH, SOD andintracellular ROS were measured respectively.The results and conclusions are summarized as follows:(1) Reperfusion significantly induced oxidative stress injury by decreasing theviability, antioxidant capacity and increasing cell apoptosis as well as promoting lipidperoxidation and the intracellular ROS level in the endothelial cells.(2) Freezing significantly inhibited reperfusion-induced oxidative stress injury by increasing the cell viability, antioxidant capacity, bcl-2protein expression and decreasingcell apoptosis as well as decreasing lipid peroxidation and bax protein expression.In summary, freezing significantly suppressed reperfusion-induced endothelialdysfunction, which might be related to the increasing of bcl-2/bax rate.Extracorporeal liver resection and partial liver autotransplantation has been animportant kind of operation for patients with primary hepatic carcinoma. The effect offreezing has become the hot spot in the research field. The present study might providesome guidance for the time control of freezing during the operations.