| Objective:To investigate the expression of Bmi-1protein and P16INK4A protein in epithelial ovarian neoplasm with the difference of histology classifications, tumor grades and clinic stages, benign ovarian tumors and normal tissues, To discussion the role of BMI-1and P16INK4A in carcinogenesis, progression and outcomes of epithelial ovarian tumors.Methods:(1) A group of patients with complete clinical data of10normal ovarian tissue,15benign of ovarian tumors and62epithelial ovarian carcinomas were selected randomly. P-V immunohistochemistry stain of BMI-1and P16INK4A was carried out on these samples. Then study the relations between the expressions of BMI-1and P16INK4A and clinical pathological characteristics.(2) Collected into the group of62examples of epithelial ovarian cancer took the surgery date as the beginning follow-up date, in October,2012for end of the follow-up date. In the follow-up the position time was27months.Results:(1) The positive expression of BMI-1protein was mainly in the cytoplasm and nucleus, yellow or brown.The positive rate of BMI-1in normal ovarian tissues, benign of ovarian tumors and in epithelial ovarian carcinomas was separately20%(2/10),40%(6/15) and72.58%(45/62). All of the differences were significant statistically (P<0.05). The differences between normal ovarian tissues and epithelial ovarian carcinomas were statistically significant (P<0.05). The differences between benign of ovarian tumors and normal ovarian tissues were not statistically significant (P>0.05).(2) The positive rate of BMI-1in Ⅰ-ⅡHand Ⅲ-Ⅳ stage of ovarian carcinomas was separately50.00%(12/24) and86.84%(33/38). The difference has significant statistically (P<0.05). The positive rate of BMI-1in G1, G2, and G3was separately42.85%(6/14);64.71%(11/17) and90.32%(28/31). The differences were significant statistically (P<0.05). The positive rate of BMI-1in Greater omentum metastasis and without Greater omentum metastasis was separately89.19%(33/37) and48%(12/25). The differences were significant statistically (P<0.05).But BMI-1were no statistic difference (P>0.05) in the type of epithelial ovarian carcinomas and the age of the patients.(3) The positive expression of P16INK4A protein was mainly in the cytoplasm and nucleus, yellow or brown.The positive rate of P16INK4Ain normal ovarian tissues, benign of ovarian tumors and in epithelial ovarian carcinomas was separately10%(1/10)、33.33%(5/15) and62.90%(39/62). All of the differences were significant statistically (P<0.05). The differences between normal ovarian tissues and epithelial ovarian carcinomas were statistically significant (P<0.05). The differences between benign of ovarian tumors and normal ovarian tissues were not statistically significant (P>0.05).(4)The positive rate of P16INK4A in Ⅰ-Ⅱ and Ⅲ-Ⅳ stage of ovarian carcinomas was separately41.67%(10/24) and76.32%(29/38). The difference has significant statistically (P<0.05). The positive rate of INK4A in G1.G2, and G3was separately35.71%(5/14);52.94%(9/17) and80.64%(25/31). The differences were significant statistically (P<0.05). The positive rate of P16INK4A in Greater omentum metastasis and without Greater omentum metastasis was separately70.27%(26/37) and52.00%(13/25). The differences were significant statistically (P<0.05).But P16INK4A were no statistic difference (P>0.05) in the type of epithelial ovarian carcinomas and the age of the patients.(5) The expression of BMI-1and p16INK4A in epithelial ovarian cancer tissues is not relevant (P>0.05).(6) In62example ovary epithelium patient, the position life of the patients who had positive BMI-1expression was34.57months. The position life of the patients who had negative BMI-1expression was45.60months, the difference has statistics significance (P<0.05). the position life of the patients who had positive p16INK4A expression was41.52months. The position life of the patients who had negative P16INK4A expression was40.20months, the difference has not statistics significance (P>0.05).Conclusion:(1) The positive expressions of Bmi-land P16INK4A in epithelial ovarian cancers were significantly higher than those in benign ovarian tumors and normal tissues, which showed over expression of BMI-1and P16INK4A may play an important role in the development of ovarian cancer.(2) In epithelial ovarian carcinomas the expression of BMI-1and P16INK4A was correlated with FIGO stage, histological grade and BMI-1was correlated with Greater omentum metastasis. Indicated that BMI-1and p16INK4A may play the important roles in ovarian cancer genesis, invasion and in metastasis and may be expected to be a new and potential marker in the observation of epithelial ovarian carcinomas.(3) The expression of BMI-1and P16INK4A in epithelial ovarian cancer tissues is not relevant.(4) The expression of BMI-1protein can be used as independent prognostic factors in patients with epithelial ovarian cancer. |
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