Epidemiological Survey And Drug Sensitivity Study Of Chlamydia Trachomatis In Urogenital Tract Infection In Zhangjiakou Region

Chlamydia trachomatis (C. trachomatis), which can cause many diseases, is a kind of obligate intracellular parasitic microorganisms. C. trachomatis commonly infects urogenital tract and causes Non-gonococcal urethritis (Nongonococcal urethritis, NGU), resulting in a variety of clinical symptoms, bringing serious physical, psychological and economic burden to patients. At present, accompanying increasing of incidence of sexually transmitted diseases, NGU caused by C. trachomatis is one of the most common sexually transmitted diseases. There is a rising trend of urogenital tract infection caused by C. trachomatis, which has become a serious hazard to public health. Although infection of urogenital tract caused by C. trachomatis has worldwide distribution, different countries and regions have different incidence, which is associated not only with age, occupation, cultural qualities and health education level, but also with economic level, geographical location, population mobility, and other factors. Antibiotics is the major drug to treat C. trachomatis infection. In the past, the clinical cure rate by antibiotics was more than90%, but at present, there are many reports of failure of Chlamydia trachomatis infection, which may associate with drug resistance and clinical antibiotic abuse.In order to understand the distribution characteristics of urogenital infection patients of C. trachomatis and drug sensitivity to common antibiotic by clinical treatment, C. trachomatis infection status of urogenital infection patients who came from Obstetrics and Gynecology of First Affiliated Hospital of Hebei North University is studied. Methods are as follows:cervical swab specimens are collected, patients’related data such as age, occupation, place of residence, and other related information was registered. First, specimen is screened by multimedia microscopy, and then PCR is used to detect the specimen to determine whether there are C. trachomatis infection or not. After that, epidemiological and statistical analysis are carried on. PCR testing positive samples are cultured on monolayer HeLa cell. C. trachomatis strains are isolated and preserved; Common antibiotics such as azithromycin, clarithromycin, doxycycline, minocycline, moxifloxacin and levofloxacin are selcted to determine antibiotic sensitivity of C. trachomatis strains. Minimum inhibitory concentration (MIC) is determined by method previously reported, so antibiotics sensitivity of C. trachomatis strains is clear.In the study, there are25positive sample cases in1183specimens with PCR testing, the positive rate being approximately2.11%. Epidemiological and statistical analysis on infection factors is made and here are the results:age is an important factor. The incidence of sexually active women is higher than those of other ages. Occupation is also an important factor. The rate of infection of service employees and self-employed is higher than cadres and civil servants. Contraceptive measure by condom has more protection compared with other methods of contraception. In addition, there are no significant differences between urban and rural areas. There is no statistical significance in specimens the collection places analysis, the positive rate of detection of vaginal secretions and cervix significant difference does not exist.MIC values (μg/ml) of6kinds of antibiotics to25C. trachomatis strains, are determined were show below:azithromycin0.063～0.500; clarithromycin0.004～0.064; doxycycline0.063～0.500; minocycline0.002～0.016; moxifloxacin0.008～0.120; levofloxacin0.031～0.500. All strains are sensitive to tested antibiotic and the C. trachomatis inclusion bodies has changed. The shape of them also changes with drug dose increasing, the shape changes from oval to irregular ones.The present study helps to understand the characteristics of C. trachomatis infection in Zhangjiakou. The results suggest that common antibiotics still can be used. The methods of culturing C. trachomatis and clinical strains from genitourinary tract infected by C. Trachomatis have been explored. A set of methods about C. trachomatis susceptibility research which is suitable for our laboratory have be successfully found. All strains are preserved, which lays a foundation for further related research of C. trachomatis.