Phenomena Of Abdominal Obesity’s Impact On Organism And Its Association With Serum Biochemical Indexes And CETP Gene Polymorphism

Background:Abdominal obesity is an independent risk factor for many diseases, and hashazards on human health. In this study, firstly, through the Type2diabetes mellitus andcoronary heart disease（CHD）, we are about to verify the accuracy and sensitivity ofAbdominal girth index (AGI) to measure abdominal adipose condition. Then to analyzewhether abdominal obesity is a risk factor for lung cancer and liver cancer by measuringAGI. To explore the influence of genetic factors on abdominal obesity throughcombined analyze the family genetics investigation of waist circumference (WC) andthree allele polymorphisms of CETP gene. And observe the changes of laboratoryindicators of different AGI groups, trying to find potential hazards of abdominal obesityon the health status and possible associations among genetic factors, abdominal obesityand laboratory indicators.Methods:1. Case-control experiments：Sixty patients were separately selected in Type2diabetes mellitus group and CHD group, while fifty individuals with lung cancer andfifty individuals with liver cancer were selected as lung cancer group and liver cancergroup, respctively. Age and sex of physical examination individuals in control groupwas one-to-one correspondent with case group. Height, weight, and abdominalcircumference were accuratly measured, and then the body mass index (Body MassIndex, BMI) values and AGI value in each group would be calculated.2. Family genetics investigation of abdominal obesity: To explore the relationshipof waist circumference (WC) between parents and children, the WC was investigated in100cases of one-child families (50boys and50girls).3. Thirty of volunteers (15males and15females) with the highest AGI were selected as the higher AGI group and thirty volunteers (15males and15females) withthe lowest AGI were selected as the lower AGI group from total100healthy volunteers(50males and50females). A fasting blood sample was collected for CETP genepolymorphism analysis by PCR and determination of serum biochemical indexes.4. Analyzing the relationship between abdominal obesity and total antioxidantcapacity (TAC): Serum TAC of higher AGI group and lower AGI group was detectedby KMnO4microtitration, Iodine microtitration and T-AOC kit.Results:1. Results of case-control experiments:Compared with the control group, the height、weight and BMI indexes in Type2diabetes mellitus group and CHD group have no significant differences（p＞0.05）, butthe indexes of WC and AGI was significantly increased（p＜0.05）.Compared with the control group, the height，weight and WC in lung cancergroup were no significant differences (p＞0.05). The BMI was significantly less thanthe control group（p＜0.05） and the AGI was significantly greater than the controlgroup （p＜0.05）.Compared with the control group, the height、weight、WC and BMI in livercancer group were no significant differences (p＞0.05), and only AGI wassignificantly increased（p＜0.05）.2. Family genetics investigation of WC:Child’s AGI may be mainly affected by father’s AGI, and likely has little to do withthe mother’s AGI. It seems that children’s AGI are mainly affected by father’s AGI, buthas little significance with the mother’s AGI. Regression equation: Child’s AGI=0.456×father’s AGI+0.532；Boy’s AGI=0.630×father’s AGI+0.371；Girl’s AGI=0.439×father’s AGI+0.528。There were no significant association between children’s BMI and parents’ BMI (p>0.05).3. Results of gene and serum biochemical indicators:Cholesterol， Triglyceride， Low density lipoprotein-cholesterol， Alanineaminotransferase，Lactate dehydrogenase，A/G，Creatinine，Uric acid，Glucose and9serum biochemical indicators in higher AGI group were significantly higher thanlower AGI group (p <0.05), while High density lipoprotein-cholesterol and Globulinwere significantly decreased (p <0.05).The frequency of the B2, V and G minor alleles in the lower AGI group was significantly higher than higher AGI group (p <0.05);The AGI values of G allele non-carriers were significantly higher than those of Gallele carriers and the results for the B2allele and V allele were very similar to G allele(p <0.05).The association between CETP gene polymorphism and BMI was not observed (p>0.05).The level of AST（p＜0.05）in V allele non-carriers were significantly higher thanthose of V allele carriers. Compared with G allele non-carriers, levels of UA and TG inG allele carriers were significant elevated (p <0.05), while concentration of HDL-C wassignificantly decreased (p <0.05).4. Antioxidant capacity results:Compared with the lower AGI group, serum TAC detected by KMnO4microtitration (p <0.05), Iodine microtitration （p＜0.05） and TAC kit （p＜0.05） inhigher AGI group were significantly reduced.Correlation analysis showed that statistical significance between the serum totalantioxidant capacity and AGI (r=-0.631, p＜0.05) and BMI (r=-0.436, p＜0.05).Conclusion:1. AGI is a good anthropometric indicator for the assessment of abdominal adipose;Increased AGI may be an independent risk factor for lung cancer and liver cancer, andmay be used as a predictor of related diseases in the future.2. Child’s WC may be mainly affected by father’s WC, and has little to do with themother’s WC.3. CETP gene polymorphism may be important intrinsic factors that lead toabdominal obesity, and will be accompanied by changes of a number of serumbiochemical indexes.4. Both BMI and AGI showed a significant negative correlation with body totalantioxidant capacity and AGI had a higher correlation coefficient, which suggest thatabdominal obesity have a greater impact on the body’s antioxidant capacity.