Research On The Function Of IL-17, IL-23and TNF-α In The Pathogenesis Of Guillain-barre Syndrome

ObjectiveGuillain-Barre syndrome (GBS) is an acute inflammatory demyelinating polyneuropathy of peripheral nerves. Until now, the exact mechanism of GBS pathogenesis is elusive. Growing evidence has shown that CD4+T cells and related cytokines play an important role in the pathogenic mechanism of GBS. The aim of this study was:(1)to investigate the function of interleukin-17(IL-17), interleukin-23(IL-23) and tumor necrosis factor-a (TNF-a) in the pathogenesis of GBS by comparing serum level of IL-17, IL-23and TNF-a between pediatric patients and heathy controls;(2)To analyze the influence of intravenous immunoglobulin(IVIG)on GBS by comparing the serum levels of IL-17, IL-23and TNF-a before and after IVIG in GBS patients.MethodsThis was a prospective study.38pediatric patients hospitalized in our department with GBS from Jan.2005to Apr.2012were prospectively divided into two groups, patients in group A were received IVIG within7th day after GBS onset.Patients in group B received IVIG during8th-12th day after GBS onset. The controls was25heathy children.All the objects were screened before study. The first5ml of venous blood was collected into tubes containing EDTAK2at7am.IVIG was injected at the dose of0.4gkg-1·d-1for5days. The second5ml of venous blood was collected into tubes containing EDTAK2at7am after IVIG treatment.The serum levels of IL-17, IL-23and TNF-a were detected by ELISA before and after IVIG treatment. All statistical analyses were performed using SPSS17.0software. The description of the measurement variables using mean±tandard deviation.The general demographic data were compared between GBS pediatric patients and healthy controls using Two independent sample t-test and;x2tests.The sample mean between two group t test is compared using Two independent sample t-test..P<0.05for the difference was statistically significant.ResultsThe serum level of IL-17, IL-23and TNF-α in pediatric patients with GBS was significantly higher than that of normal control (P<0.05). After treatment of FVIG, the serum level of IL-17, IL-23and TNF-αin pediatric patients with GBS became obviously lower than that before treatment (P<0.05).There were no significant difference between the two groups before and after treatment(.P>0.05).ConclusionsThe serum level of IL-17, IL-23and TNF-α pediatric patients with GBS rose in acute stage, which indicating the important role of cytokines in the development of GBS. IVIG treatment could restrain the secretion of IL-17, IL-23and TNF-α, thus alleviate cellular inflammatory response of patients with GBS.