Invertigation Of Effects Of EGB On Rats With Liver Cirrhosis And Portal Hypertension And Its Mechanisms

Objective: To observe the effects of EGB on liver function、pathological changes of liver、PHT and expression of VEGF、iNOS inliver tissure of rats with liver cirrhosis and portal hypertension. Toexplore the mechanism of EGB in the treatment of liver cirrhosis andPHT from VEGF、iNOS angle. Methods:6rats were selected randomlyfrom36male SD rats as the normal control group(group N). Cirrhosiswas induced in remaining30rats by a complex method of injectioncarbon tetrachloride(CCl4).Comfirmed the success of modeling,cirrhosis rats were randomly assigned into3groups：cirrhosis modelgroup(group M),low dose EGB treated group(group L),high dose EGBtreated group(group H). Group L、Group H were respectively given100mg/kg,200mg/kg of EGB by daily gavage,6weeks.Group M、Groupe N were given the same volume of0.9%N.S at the sametime.After finishment of treatment，rats were anesthetized by abdominalinjection，and we applied scale measurement measured portal venouspressure of every rats.ALT、AST、TBIL、TP、ALB、GLO、A/G of everyrats were measured.NO in every rats serum were measured by nitralereduetase;Observed liver tissue of each group rats under the lightmicroscope and according to the score of Ishak liver disease and correction scheme of histopathological lesions were inflammatoryactivity and the degree of liver fibrosis score on sections of hepatictissue of rats;VEGF、iNOS protein expressed in liver tissure weremeasured by SP.At last,all the results were analyzed by using SPSS17.0software. Results:1.The degrees of liver inflammation and hepaticfibrosis of groupe M、L、H were significantly higher than groupeN(P<0.05);Groupe L、H of liver inflammation and fibrosis degree werereduced to group M(P<0.05)，and groupe H was more reduced comparedwith groupe L(P<0.05).2. ALT、AST、TBIL、NO of group M、L、Hwere more than group N(P<0.05);ALT、AST、TBIL、NO of group L、H were less than group M(p<0.05);group H was less than groupeL(P<0.05)；3.TP、ALB、A/G of groupe M、L、H were less than groupeN(P<0.05)；ALB、TP、A/G of groupH、L were more than groupeM(p<0.05)；4.Potal venous pressure of groupe M、L、H were higher thangroupe N(P<0.05); Potal venous pressure of groupe L、H were lowerthan groupe M (P<0.05);groupe H was much lower than groupeL(P<0.05)；5.iNOS protein expressed in liver tissure of groupe M、L、H was higher than groupe N(P<0.05); iNOS protein expressed in livertissure of groupe L、H were lower than groupe M(P<0.05)；groupe Hwas much lower than groupe L(P<0.05)；6.VEGF protein expressed inliver tissure of groupe M、L、H was higher than groupe N(P<0.05);VEGF protein expressed in liver tissure of groupe L、H were higher than groupe M(P<0.05); groupe H was more higher than groupe L(P<0.05)。Conclusion：1.NO which is induced by iNOS can promote livercirrhosis and PHT development；2.VEGF can delay the development ofliver cirrhosis and PHT；3. EGB can improve liver function、 lighten thedegree of liver inflammation and fibrosis, reduce the PVP,the treatmenteffect of H groupe is better than L groupe；4.EGB could depress theexpression of iNOS protein in liver tissues and reduced the production ofserum NO, increase the expression of VEGF protein in liver tissues ofrats with liver cirrhosis and portal hypertension,which may be one of itsmechanisms of action.