The Expression Of MT1-MMP And RECK In The Endometrial Tissue Of Women With Adenomyosis And The Ralationship Of Both Proteins With Angiogenesis

Objective: We detected the expression of membranetype-1matrix metalloproteinase (MT1-MMP) andreversion-inducing-cysteine-rich protein with kazal modifs (RECK) inectopic endometrial, eutopic endometrium of adenomyosis and controlendometrium, we also analyzed the relationship of RECK,MT1-MMP andangiogenesis in AM by detecting the microvessel density in ectopicendometrial, eutopic endometrium and control endometrium, and weaimed to explore the role of these proteins in pathogenesis of AM.Methods: We collected ectopic endometrium and eutopic endometriumfrom35patients with AM, and collected30cases of control endometriumfrom the patients with hysterectomy uterus because of myoma. All of thepatients had not been treated with the hormone and anti-endometriosismedication six months before operation. The expression of RECK andMT1-MMP was measured by immunohistochemistry, and the microvesseldensity (MVD) was detected by the immunohistochemistry staining ofCD34. The statistical methods were carried out by the SPSS17.0softwareand P<0.05was considered as representing significant differences.Results:1. The expression of MT1-MMP in ectopic endometrium wasincreased, compared with eutopic endometrium and control endometrium (P<0.01, P<0.01). The expression of MT1-MMP in eutopic endometriumwas also increased, compared with the control group(P＜0.01).2.Theexpression of RECK protein in ectopic endometrium was decreased,compared with eutopic endometrium and control endometrium (P<0.05,P<0.05). The expression of RECK in eutopic endometrium was alsodecreased, compared with the control group， but there wasn’tcorrelation(P＞0.05).3. The MVD in ectopic endometrium was higher,compared with eutopic endometrium and control endometrium (P<0.01,P<0.01). The expression in eutopic endometrium was also increased,compared with the control group(P>0.05).4. In ectopic endometriotictissue, eutopic endometriotic tissue and the control group, there wasnegative correlation between RECK and MT1-MMP（P<0.01, P<0.01,P<0.01）. MT1-MMP and MVD also showed a positive correlation inectopic and eutopic endometriotic tissue（P<0.01, P<0.01）. There wasn’tcorrelation between MT1-MMP and MVD in control group (r=0.353, P＞0.05). There was negatively correlated between RECK and MVD inectopic and eutopic endometrium（P<0.05, r＝－0.352; P<0.05, r＝－0.386）. There was no correlation between RECK and MVD expression incontrol group(r=－0.197, P＞0.05). Conclusion:1. MT1-MMP andRECK were negatively correlated in the three groups, and the resultshowed the relationships of negative feedback and mutual antagonismbetween the two proteins. In addition, In the three groups of endometrial tissue, MT1-MMP had the highest expression and RECK had the lowestexpression in ectopic group, the results may proved the relationships ofnegative feedback and mutual antagonism between the two proteinswhich were obviously existed in ectopic endometrium. We speculatedthat the highest expression of MT1-MMP in ectopic endometrium brokethe balance of the two proteins and decreased the expression of RECK;the low expression of RECK feedback to promote the further higherexpression of MT1-MMP, and promote the development of AM.2.MT1-MMP and MVD also showed a positive correlation in ectopic andeutopic endometriotic tissue. But there wasn’t correlation in controlgroup.The result showed that MT1-MMP may have the effect ofpromoting blood vessel growth in the development of AM.3.RECK andMVD also showed a negative correlation in ectopic and eutopicendometriotic tissue and no correlation in control group.The resultshowed that RECK inhabited the process of angiogenesis in AM.