The Effect Research Of NF-κappa B In The Treatment For Infantile Hemangioma In Nude Mice Model With Propranolol

Objective: To research the expression of nucleartranscription factor kappa B、kappa B inhibitory factor and B-cellLymphoma-2in the treatment of infantile hemangioma in nude micemodel with propranolol and discuss the possible mechanism ofpropranolol to hemangioma. Methods: Under the sterile condition，wesurgically removed of a hemangioma from a four month old female infantin proliferation phase. Sample was divided into small pieces andtransplanted into the nude mouse subcutaneously. Then hemangiomaanimal model was established successfully.50mice which hemangiomasurvived in were randomly divided into two groups: propranolol groupand saline solution group (control group). We daily observed the shapeand volume of transplanted tumors. Before the treatment and on the7th、14th、21th、28th day after the first intervention, every5nude mice had berandomly killed by cervical dislocation in every group for test.Hemangioma samples were measured and tested by HE stainingimmunohistochemistry and reverse transcription PCR. The samples oftransplanted tumor were tested for NF-kappa Bp65、IκB-α and Bcl-2byimmunohistochemical method. The expression of NF-kappa Bp65mRNAin samples was tested by semi-quantitative RT-PCR. Results:1.specimen and HE staining：after intervention, the transplanted tumors in propranolol group became smaller, lighter and harden. Hemangiomaendothelial cells decreased significantly in propranolol group，and therewere a lot of fibro-fatty tissue. But in saline group，the transplantedtumors grew up firstly on the7th、14th day and appeared lots ofhemangioma endothelial cells. Then on the21th、28th day，tumor tissueinvolved. Hemangioma endothelial cells decreased dramatically and therewere a lot of fibrous tissues. At every time point，the tumors in twogroups was different apparently (P<0.05).2. Immunohistochemistrydetection: at every time point，the NF-κBp65in propranolol group waslower than that in saline group(P<0.05); IκB-α in propranolol groupshowed was higher than that in saline group (P<0.05); the Bcl-2inpropranolol group was lower than that in saline group (P<0.05). At thesame time, there was a linear and positive correlation between NF-kappaBp65and Bcl-2in propranolol group (r=0.901，P<0.01).3. Sq-RT-PCR：In each phase, NF-kappaBp65mRNA in propranolol group wassignificantly lower than in saline group(P<0.05). Conclusions:1. Thepropranolol had obvious therapeutical effect in infantile hemangioma innude mice model.2. Propranolol rised the expression of the IκB-α andreduced the expression of NF-κBp65and Bcl-2in nude mice model.3.Propranolol treating infantile hemangioma in nude mice model mightweaken the anti-apoptotical effect mediated by the NF-kappa B onhemangioma endothelial cells.