Primary Study On The Expression Of Protein Aiginine Methyltransferase4(PRMT4) And Asymmetric Dimethylarginine (ADMA) On Polycystic Ovary Syndrome

Objective: To investigate the level of asymmetricdimethylarginines(ADMA) in serum of women with and without polycysticovary syndrome(PCOS),as well as the PCOS model rat and controlgroup,and the expression of protein aiginine methyltransferase4(PRMT4)in the ovarian tissues of PCOS model rats and control cases.Methods: Enzyme linked immunosorbent assay was used to investigatethe level of ADMA in serum of49PCOS patients and20normal patients，also the20PCOS model rats and20normal rats. The expression of PRMT4in ovarian tissues were detected by Sp immunohistochemical method(including20PCOS model rats and20control cases).Results:1. In human serum, ADMA concentrations was0.49±0.35μmol/L in PCOS group,and was0.26±0.09μmol/L in the control group.There were significant differences in the two groups (P <0.01).2. In ratserum, ADMA concentrations was0.55±0.14μmol/L in PCOS group, andwas0.23±0.08μmol/L in the control group. There were significant differences in the two groups (P <0.01).3.In rat ovarian tissue, PRMT4IODwas44.96±9.40in PCOS group, and was18.27±3.83in control group, thedifferences were statistically significant (P <0.01).Conclusion: in ovarian tissue,the increasing of expression of PRMT4may bring about a elevated level of ADMA in serum, which causes theendothelial dysfunction of PCOS and could affect the development ofPCOS.