Protectiv Effects Of Shuangge Jiecheng Pill On AFL Rata

Objectives: Nonalcoholic fatty liver (NAFL) is a kind of diseasecaused by accumulation of lipid over five percent of liver wet weight, is theearliest and most common alcoholic liver disease (ALD), and is also thesecond liver disease following viral hepatitis, which has been influencedhuman health for a large scale. Until now the ways to mitigate such kind ofdisease are mainly drinking stop, liver cell protecting agents andantioxidants. However, there are no effective medicines because factorsinduced this disease are complex and we have not figured them out all.Shuangge Jiecheng Pill is a new kind of traditional Chinese medicinewhich can help generate more sweat and urine to benefit wet detumescence,soothe and smooth the liver and blood based on dampness-heat syndrome.Thus, we studied the protective effects and mechanisms of ShuanggeJiecheng Pill through AFL rats modeling by ways of physiologicalpathways of intragastric alcohol.Methods: We have total of65rats,11for the control group ofgavaged distilled water,54(AFL group) for model of rats of AFL diseaseinduced by56°Hongxing wine by intragastric administration for8weeks.1 rats and4AFL rats were sampled to make pathological biopsis to makesure that AFL has been developed. Other50AFL rats were randomlydivided into6groups,10were control group,10model group,10Haiwang(HW) group and30Shuangge Jiecheng Pill (SG) group with highdose (HD), middle dose (MD) and low dose (LD) on average. SG groupwere treated with different dosages of Shuangge Jiecheng Pill(20g、10g、5g crude drug/kg d) and compared with HW group administrated byHaiwang Jinzun Tablet（1.5g/kgbw）for4weeks. The changes of serumtumor necrosis factor-α (TNF-α), lnterleukin-6(IL-6), leptin (LEP),adiponectin (ADP), superoxide dismutase (SOD), malondialdehyde (MDA),free fatty acid (FFA) and glutathione peroxidase (GSH-Px) andpathological biopsis of the rats in the different experimental groups wereobserved.Results: Compared with the control group, ADP, GSH-PX and SODin the model group was obviously decreased（P＜0.05) and IL-6, TNF-,MDA, FFA and LEP were obviously increased（P＜0.05). Compared withthe model group, ADP in the HW and SG group of HD, MD and LD wereobviously increased（P＜0.05) while MDA and IL-6,TNF-6,FFA and LEPobviously decreased（P＜0.05). ADP in SG group of HD were obviouslyincreased（P＜0.01) while IL-6,TNF-6,FFA and LEP obviously decreased（P＜0.01) than those of HW, SG group of MD and LD. After treatments,HD significantly decreased fatty degeneration of hepatic cells (P<0.05) and degeneration were also observed reducing in MD, LD rats.Conclusions: Shuangge Jiecheng Pill is an effective method foralcoholic fatty liver disease by improving the lipid peroxidation of liver andreducing the lipid accumulation and liver fatty degeneration. The effect ofSG group of HD is significantly superior to the others.