Influence Of PKC Beta Inhibitor On The Renal Chemokines After Kidney Transplantation In Mice

Objective: To explore the changes of renal interstitial chemokines and peripheral bloodcells through inhibition of PKC beta after kidney transplantation in mice and itsmechanism.Methods: For allograft kidney transplantation and isograft kidney transplant in mice,renal transplantation model is set up. Mice were randomly divided into3groups: allograftcontrol group, allograft PKC beta inhibitor treatment group and isograft control group. ByPKC beta inhibitor treatment group, the PKC beta inhibitor was by gavage1day beforeoperation, the rest two groups gavaged the equal volume of normal saline. They weresacrificed at d1postoperation. The kidneys were taken for pathological analysis. Theexpression of IL-4、IL-8、、MCP-1、MIF、LCF and LIF in the kidneys was detected by usingimmunohistochemistry and the expression of LCF、HIF-1、IL-8、、MCP-1、LIF、MIF andHO-1by RT-PCR.Results: Immunohistochemistry consequences: the expressions of IL-4、IL-8、MCP-1、MIF and LCF were increased greatly in allografts control group, however, they wereremarkable reduced in both allografts PKC beta inhibitor treatment group and isograftcontrol transplantation; The reduction in allografts PKC beta inhibitor treatment group issignificant, as compared to the allograft kidney transplantation﹙P＜0.01﹚; Comparedwith isograft control group, allograft control group had been increased significantly﹙P＜0.01﹚. The expression of LIF was raised clearly both allografts PKC beta inhibitortreatment group and isograft control group, but there was remarkable reduction inallografts control group; There was significant increase in allografts PKC beta inhibitortreatment group as compared to the allograft control group﹙P＜0.01﹚, allograft kidneytransplantation had been reduced obviously compared with isograft control group﹙P＜0.01﹚. RT-PCR results: the expression of IL-8、MCP-1and LCF were increased greatly in allografts control group, however, there were remarkable reduction both allografts PKCbeta inhibitor treatment group and isograft control group; allografts PKC beta inhibitortreatment group had been reduced obviously compared with allografts control group(P＜0.05),there was remarkable increase in allografts control group as compared to the isograftcontrol group﹙P＜0.05﹚. There was the remarkable expression of HIF、MIF and LIFboth allografts PKC beta inhibitor treatment group and isograft control group as comparedto the allografts control group; Compared with the allograft control group, there wassignificant increase in allografts PKC beta inhibitor treatment group﹙P＜0.05﹚,allograft control group had been reduced obviously compared with isograft control group﹙P＜0.05﹚. The level expression of HO-1was significantly reduced both the PKC betainhibitor group and allograft control group﹙P＜0.05﹚, which was raised clearly in theisograft control group﹙P＜0.05﹚.Conclusion: The results suggested PKC beta inhibitor can reduce the infiltration of renalchemokines and improve the level expression of inflammation inhibiting factors afterkidney transplantation, thereby preventing cell infiltration by reducing the expression ofinflammatory chemokines and alleviating renal inflammation by increasing the expressionof inflammation inhibiting factors, and protecting the kidney．...