The Change Of Notch Signaling Pathway In Mice CHD Model With QDBS Syndrome And The Study Of Intervention Effect Of Shenshen Kangxin Capsule

Background: CHD is a global human heath threat with high incidence and death rate. Inrecent years, domestic CHD incidence is increasing year by year. Modern medicine has madegreat progress in the diagnosis and treatment of CHD, but there still some problems: themechanism of coronary heart disease is not clear; lack of Specific marker for early diagnosisand different stages of CHD; there are some problems and local in the treatment for CHD.TCM treatment for CHD has advantages in individual treatment, Symptom improvement andenhance live quality, also got some progress in angiogenesis. Qi-deficiency and blood-stasissyndrome is one of the most common types of syndrome differentiation of TCM for CHD.Notch signaling pathway play an important role in development and differentiation of theheart and blood vessels, also closely related to the occurrence and development ofcardiovascular diseases: damage control in ischemia and hypoxia response, angiogenesis,myocardial regeneration and repair, inhibition of myocardial fibrosis in the process of thispathway has an important role, has become a research focus in the field of cardiovascular atpresent. The researches about Notch signal pathway in CHD and CHD with Qi-deficiency andblood-stasis syndrome is little. Carry out research about the correlation between Notch signalpathway and CHD with Qi-deficiency and blood-stasis syndrome has certain theoretical andrealistic significance. Objective: To observe the expression quantity of Notch signalingpathway related genes in CHD QDBS mice model, reveal the correlation between Notchsignal pathway and CHD with QDBS syndrome; based on the theory of correspondence ofprescription and syndrome in TCM, observe the intervention effect of Shenshen kangxincapsules, explain the changes of Notch signal pathway in CHD with QDBS further.Methods:Experiment mice was random allocation to six groups. Each groups were given differentdrugs pre intervention, and copy the model of qi-deficiency and blood-stasis, except the blankgroup. After the QDBS syndrome model were over, intraperitoneal injection of Pituitrin tocopy the model of myocardial ischemia. Used electrocardiograph and QDBS syndrome Objective scale to verify the model. Expression of Notch in myocardial tissue of mice wasdetected in RT-PCR related receptor, ligand, gene, agarose gel electrophoresis and the resultswere semi quantitative analysis, IBM Spss Statistics19was used to statistical analysis.Results: Compared to the normal group, the expression of myocardial tissue in micemodel of Notch2, Notch3, Hes1, Hey2, Jagged1RNA increased, and the difference wassignificant (P <0.05);Notch1, Notch4, Hes5, Hey1RNA expression showing a cleardownward trend, and there is statistical significance. Compared with model group, theconsistency of drug intervention group in improving the expression of Notch1-3, HEY1-2,Jagged1gene, and the difference has statistical significance. Compared with the positivecontrol group, Chinese medicine group has advantages in regulate the expressions of Nothc1、Hey1, Hey2, Jagged1. Conclusion: Notch gene’s expression are abnormal in the mousemodel of CHD with QDBS syndrome, Notch signaling pathway may be involved in thedevelopment of myocardial ischemia. Shenshen kangxin capsules has a certain regulation inCHD with QDBS syndrome, the mechanism may relate to regulate the expression of Notchsignal pathway.