Shi SiWeiRongJieTang On Rats With Hyperplasia Of Mammary Gland Serum Estradiol And Progesterone Levels Of Intervention Research

Objective: Shi SiWeiRongJieTang is developed by Professor Liang with hisexperices to treat HMG for many years,and collect ancient prescriptions and theuse of the frequency statistics.Which formed with forteen kinds of TraditionalChinese Medicine,for example Radix Bupleuri,Radix Paeoniae Rubra, RadixCurcumae, Corydalis Tuber,Pangolin Scales, Cornu Cervidegelatinatum,Seaweed,Kelp,Green Tangerine peel,Angelica root.ect.The efficacyis dissipate bump to relieve pain and activating blood,remedy theswelling,nodules,pains, Clinical manifestations illness of local Breast,The effectis significant. Subject to the guidance of TCM basic theory, Designed tostudy experimentally, Observation education Shi SiWeiRongJieTang aim tothe change of the rats’ Histomorphological, Nipple height and diameter,thecontens of Estradiol,Progesterone through the model of HMG rats.Experimental Studies on the Method of Promoting Flow of Qi and BloodCirculation and Resolving the Hard Lumps, Discussion the mechanism of thisChinese prescription to cure HMG.Provide more open for treatment of HMG withTCM research and treatment basic foundation, Provide scientific reference forfurther study and clinical efficacy Further.Methods: This thesis selects the80day old male Wistar rats60only as theobject of study,weight180g-200g, The blank group intramuscular injectionof normal saline at the same dosage, The remaining rats intramuscularinjection of Estradiol0.5/kg/d,for20consecutive days,then intramuscularinjection of progesterone after5mg/kg/d, for5consecutive days,Copy themodel of hyperplasia of mammary glands in rats. To observe the changes ofrat papillary shape after25days,compared with the black group randomly,tocheck the model is successful. Rats were randomized into6groups:blank group,model group,Shi SiWeiRongJieTang high dose group（high dose group）, ShiSiWeiRongJieTang and moderate dose group（moderate dose group）, ShiSiWeiRongJieTang low dose group（low dose group）,Ru PiXiao group（the positivecontrol）,ten rats every group. In the same feeding conditions, while model theHMG rats, black group and model group give the same dose of distilled water, lowdose group1ml/100g（1.5g tcm） use Homemade prescription,once a day, for30consecutive days; The moderate dose group1ml/100g（3g tcm） use Homemadeprescription,once a day, for30consecutive days; The high dose group1ml/100g（6g tcm） use Homemade prescription,once a day, for30consecutivedays; The positive control use Ru PiXiao0.5g/100g dissolve the powder withdistilled water to2ml, once a day, for30consecutive days.30days later, ratsanesthetized with urethane, Take the pair2of breasts and stuffed, Observe themorphological characteristics of breast under the electron microscope, Estradioland Progesterone were detected by the method of radioimmunoassay.Results:1The effect of therapeutic of Shi SiWeiRongJieTang on HMG ratsweight: The weight of Normal group grow naturally, model group growslowly,compared with model group, Ru PiXiao group, Shi SiWeiRongJieTang highdose group, Shi SiWeiRongJieTang and moderate dose group weights increasedsignificantly（P＜0.01）.2All rats nipple height and diameter of Shi SiWeiRongJieTang lowercompared with model group,the results of high dose group,and moderate dosegroup notability（P＜0.01）.3Effects of pathological morphology on hyperplasia of mammary glands inrats:The results showed that compared with blank group, Hyperplasia of the Glandalveolus and ductus was in great number. Gland alveolus in lobule of mammarygland was intensive. and inequality of size. Obviously,there was expansion inductal cavity and acinar cavity. And so much secretion was in intracavity.Compared with model group,in Shi SiWeiRongJieTang changes were significantlyimproved,Ru PiXiao group, high dose group,and moderate dose group notabilityexpansion in ductal cavity and acinar cavity was not obvious The secretion waslittle. There was no significant improvement in low dose group.4The effect of Shi SiWeiRongJieTang on E₂and P in blood serum of ratwith hyperplasia of mammary glands: Shi SiWeiRongJieTang can decrease thecontents of Estradiol, high dose group which had statistical significance（P＜ 0.01）.Increase the contents of Progesterone, The high dose group which hadstatistical significance（P＜0.01）.Conclusion:1Shi SiWeiRongJieTang improve the emotion of rats,whet theappetite, promote weight gain.2Shi SiWeiRongJieTang can reduce the diameter of mammary and theswelling of the breast of the rat’s HMG modle.3Shi SiWeiRongJieTang can improve the pathological morphology of ratwith hyperplasia of mammary glands,which prove that this Prescription canimprove and protect the breast in a certain degree.4Shi SiWeiRongJieTang down-regulats the increasing Estradiol ofmammary gland and increases the degrading Progesterone of mammary gland.