Oxidative Burst And Phagocytosis Of Circulating Neutrophils Following Traumatic Brain Injury In Human

Objectives:To investigate the change of oxidative burst and phagocytosis of circulating neutrophil of patients after early traumatic brain injury.Methods:12patients with brain injury (Traumatic brain injury group, TBI),12fracture patients (Trauma control group, TC) and10healthy person (Uninjured group, U) were constituted the three goups. Oxidative burst, phagocytic rate, free radical, NF-kB, gp91phox, COX-2, iNOS, TNF-α, JL-6and CRP were detected at6h,12h,24h,24h,48h,72h, lw and2w after injury in peripheral blood neutrophils or in plasma.Results:The Oxidative burst in TBI group at each time point were significantly higher than in U group (p<0.05), moreover, the TBI group were more higher than the TC group; Phagocytic rate in TBI group were significantly lower at each time point and in TC group were significantly lower at24h-72h (p<0.05), however, the phagocytic rate in the TC group would return to normal after1w, and in TBI group the rate was at a low level in the2weeks; Free radical increased significantly in TBI and TC groups at almost all the time points assessed (p<0.05), except after2weeks for the trauma controls, while, the more serious brain injury, the more increased; The gp91phox, COX-2and iNOS were all significantly increased in TBI and TC groups24hours after injury (p<0.05), moreover, the TBI group were more higher than the TC group. However, the increases of NF-kB were similar in both TBI and TC groups. Cytokines TNF-α, IL-6and CRP in the TC group would return to normal after1w, and in TBI group they were at a high level in the2weeks (p<0.01).Conclusion:Increased oxidative burst and decreased phagocytic rate of circulating neutrophils were confirmed in patients with early brain injury.