Inflammatory Cytokines In Patients Receving Autologous Transfusion After Total Hip Replacement

【Objective】 To investigate Inflammatory cytokines of autologous orallogeneic transfusion in total hip replacement patients.【Methods】20total hip replacement patients under general anesthesia wererandomly divided into autologous group and allogeneic group(n=10).The patients inallogeneic group only receive allogeneic transfusion.The patients in autologousgroup predeposite autologous whole blood400ml before operation.They alsotransfuse intraoperative salvage of shed blood.Plasma IL-6、IL-8、TNF-α、IL-4、IL-10were measured immediately after anesthesia （T1）,before transfusion（T2）,after transfusion（T3）,on the1st（T4）,4th（T5）and7th（T6）day afteroperation by ELISA.Blood routine test were also recorded on the1st,4thand7thdayafter operation.【Results】Postoperative Hb decresed obviously(P＜0.05),and postoperativeWBC increased obviously in all patients of two groups（P＜0.05）,.On the4thdayafter operation,WBC of autologous group increased more significantly than that ofallogeneic group (P=0.048). Comparing with T1, IL-6of allogeneic group increasedsignificantly (P <0.05) on the1st,7thday after operation,IL-6of autologous groupincreased significantly (P <0.05) on the1st,4thand7thday after operation; IL-8ofautologous group increased significantly (P <0.05) on the1stday after operation;IL-10of autologous group and allogeneic group increased significantly (P <0.05)on the1st,4thand7thday after operation. On the4thafter operation,IL-8ofautologous group was higher than that of allogeneic group（P=0.048）,but IL-4waslower than that of allogeneic group（P=0.045）. 【Conclusion】 Predeposited autologous whole blood associating withintraoperative autologous blood can reduce the usage amout of allogeneic blood andsave blood resources,but we only find that on the4thafter operation,,IL-8ofautologous group was higher than that of allogeneic group,IL-4was lower than thatof allogeneic group,so the results suggest further researches are needed to carry outabout the immune function of intraoperative autologous blood.