| Objective: To investigate the effect on proliferation and metastasis in rat primaryhepatocelluar carcinoma (HCC) by AMD3100, a specific antagonist of CXCR4, and toexplore the mechanism basically.Methods:64rats were randomly divided into group A (control group of32rats)with primary liver cancer SD model and group B (treatment group of32rats), of which theprimary liver cancer model in rats were set up by chemical reagent through the way ofgavage, intraperitoneal injection and partial nephrectomy of liver. In the early liver cancerstage, AMD3100was used to treat rats in group B by intraperitoneal injection. After20weeks, all rats were killed, the livers and the hepatic portal have been taken out completely.The mortality measurement between groups of rats and weight changes have been recorded,the final liver tumor sizes have been measured and the hepatic portal lymph node metastasisbeen counted too. Through HE staining, the tissues’ pathological changes have beenobserved. The tumor microvascular density has been observed with the microscope,immunohistochemical (IHC) method has been used to detect the expression of VEGF,MMP-9, and CD133of each rat’s cancerous tissue. The statistical process was carried outwith the statistical software of SPSS17.0, thus the results achieved.Results:(1) The SD rat mortality of group A and B is28.1%,6.3%, respectively,which has a statistical significance(P<0.05). During the treatment process, the weight ofgroup A is significantly lighter than group B.(2) the general to the naked eye of tumorformation rate in group A SD rats was observed, the surface of the liver cancer and livercancer surface diameter, respectively100.0%、16.43±4.52、7.86±1.91mm, were greaterthan group B70.0%、6.43±1.54、2.03±0.73mm, is similar between the two groups have statistical significance (P <0.05).(3) The hepatic tumor metastasis rate of portal for A, Bgroup is21.7%,3.3%, respectively, with statistical significance (P<0.05).(4)The tumormicrovascular density (MVD) count between two groups is25.85±6.14and8.08±2.47,respectively, has a statistical significance (P<0.05).(5) IHC results show that the VEGF,MMP-9, CD133average integral optical density value of cancerous tissue in group A is338.32±41.67、284.69±51.17,、338.32±21.43, which is significantly higher than group B120.93±13.10、104.69±11.17、133.68±19.30, the differences were statistically significant(P<0.05).(6) The Real-time PCR results show that the VEGF, MMP-9, CD133averageintegral optical density value of group A is98.32±16.67、84.69±15.17、105.45±21.32,which is much higher than group B40.76±12.52，43.9±9.37，61.68±12.33, the differenceshave statistical significance (P<0.05).Conclusion:1、AMD3100can inhibit the growth and metastasis of primary livercancer on rats.2、Inhibition of rat primary tumor growth and metastasis by AMD3100maythrough the way of down regulating the expression of VEGF and MMP-9.3、AMD3100has an inhibitory effect on rat primary liver cancer stem cells. |
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