The Monitoring Of Human Typical Pollutants Internal Exposure And Human Genetic Damage Effect In A Region Of Huaihe River Basin

Objective：It was still unexplained of the high incidence of cancer in parts of Huaihe Riverbasin. While the complex exposure to environmental factors, it was too difficult to explorethe etiology of cancer. According to those local typical pollutants which have been screenedby research, Carrying out monitoring of internal exposure and effect indicators would helpto capture the internal exposure levels of the main typical pollutants of people, which couldprovide a scientific basis for the evaluation of health risks caused by typical pollutants forlocal residents and specify direction of local environmental pollution control.Methods：On the basis of comparative study methods, a control area and four monitoringareas were selected in the territory of pilot region. Samples were the morning urine of localordinarily residents, of which the heavy metals,1-hydroxypyrene, nitrosamines and8-hydroxy deoxyguanosine would be monitored and tested respectively. Among them, theheavy metal detection used Inductively Coupled Plasma-Mass Spectometry while1-hydroxypyrene was detected by Ultra Performance Liquid Chromatography-MassSpectometry, nitrosamines were detected by Gas Chromatography-Mass Spectometry while8-hydroxy deoxyguanosine detection used High Performance LiquidChromatography-Electrochemical method. Results： All of the eight kinds of monitoring heavy metals were detected in the urinesamples. The chromium and lead levels in urine of the four monitoring areas weresignificantly lower than the control area while the beryllium in urine of two monitoringzones was significantly higher than the control area.1-hydroxypyrene levels in urine had nosignificant difference in the monitoring areas and control range, but its concentration wassignificantly higher than the level of the general population. Eight kinds of volatilenitrosamines were all detected in the urine samples and the total urinary nitrosamines levelsof the four monitoring areas were significantly higher than the control area. The8-hydroxydeoxyguanosine in urine of one monitoring zones was significantly lower than the controlarea and the rest monitoring zones had no significant difference compared with the controlgroup. Meanwhile, the concentration of8-hydroxy deoxyguanosine in the five regions wassignificantly higher than that of the general population. The concentrations of chromiumand lead in urine were inversely correlated with the gastrointestinal cancer mortality oflocal residents while selenium was significantly negatively correlated with human damageindicators. The urinary total nitrosamine content was significantly positively correlatedwith the total cancer mortality and gastrointestinal cancer mortality of local residents.Conclusions：The high concentration of1-OHP in urine stated that there was a health riskfor local people caused by the high exposure to polycyclic aromatic hydrocarbons. Urinarytotal nitrosamine content was significantly positively correlated with the cancer mortality oflocal residents, which suggested that the high incidence of cancer in monitoring regionmight be related to the exposure of nitrosamines substances. The high concentration of8-hydroxy deoxyguanosine indicated that local residents suffered a high intensity of geneticdamage, which suggested that there was a potential health threat for local people, andwhich the selenium might have a protective effect for.