Research On The Effects Of The Activities Of Learning And Memory In Rats By Low-frequency Electromagnetic Radiation

As the development of science and technology, the electromagnetic wavetechnology is widely applied in military,communication，industry，agriculture andother fields. At the same time, its negative impact can not be ignored. Nowadays, theelectromagnetic radiation pollution has become the focus of a new kind ofenvironmental pollution. Studies have shown that the high frequency electromagneticradiation may have potential danger to human body, such as the body’s circulatorysystem immune reproductive and metabolic function, the central nervous system,and the ability of learning and memory. What is more, the high frequencyelectromagnetic radiation can cause cancer and accelerate the body’s cellsproliferation. In daily life, people can avoid the high frequency electromagneticradiation damage, but these home appliance just like mobile phone and computer thatwill produce low frequency electromagnetic radiation are hard to avoid. Doeslow-frequency electromagnetic radiation harm the features of human body? Therelevant report is less.This study used adult wistar rats as the research object and discussed the typicalcell phone electromagnetic wave frequency of900MHZ, the power of10w/m2of theelectromagnetic wave radiation on rat central nervous system damage as to the impacton learning and memory. This article consists three parts. The first is the animalbehavior experiment that effects on learning and memory in rats from themacroscopic perspective analysis of low frequency electromagnetic radiation througheight walls of the maze experiment. Then we took out the important organization ofthe hippocampus of learning and memory, analyzed some important changes inneurotransmitter acetylcholine esterase activity and discussed the relationshipbetween acetylcholine and the learning and memory. Finally, through the guide morephysiological recorder and glass electrode extracellular recording techniquecomparison, we learned that the radiation conditions change of neuron discharge.Therefore, low frequency electromagnetic radiation can effect on learning andmemory in rats through the further analysis.Eight walls of the maze test results showed that the control and radiation group’experiment time, the total number of go into the arm, number of reference memoryand working memory error increased inordinately after the low intensityelectromagnetic radiation of1-10weeks. Especially in4weeks or so, the control andradiation group’ time of rat experiment takes maximum. From the phenomenon, we can see that rats in control and radiation group’ memory were declining. Four weekslater, the time of rat experiment less than six weeks before, that because of natureforgotten as well as the radiation group rats adaptability to low frequencyelectromagnetic radiation. However, the correct number before first error have notsignificant differences compared control and radiation group. Animal memory andreaction decline significantly because of low-frequency electromagnetic radiation. Butit has no obvious impacts to deep memory of the rats.Basing on acetylcholinesterase activity determination results showed that after10weeks low-frequency electromagnetic radiation, acetylcholine esterase vigorincreased by42.7%, which reduce content of acetylcholine. That could be one of thereasons that caused decrease ability of learning and memory by rats.By the experimental of extracellular recording technique results showed that:1.Electromagnetic radiation has an inhibitory hippocampus CA1region neuronsdischarge frequency role, especially when electromagnetic time is40min, whichinhibition rate achieve maximum. After electromagnetic irradiation, dischargefrequency of the hippocampus CA1region neurons is reduced, but the scale is notobvious, when up to20min, its tends to be stable.2. Electromagnetic radiation maychange the hippocampus CA1region neurons spontaneous discharge type makeexplosive discharge types increased obviously, increased from32%to47%.3.Radiation group in5min in peak potential total number has significantly reduced,maximum incubation period less than control group to the maximum incubationperiod, the large amplitude reduced.4. Using mathematical method toelectromagnetic radiation sampling sequence for correlation analysis, the thresholdprocessing to cross correlation coefficient radiation group than control group,indicating that the electromagnetic radiation between neurons is a tend to excitatoryconnection. We hypothesized that electromagnetic radiation may cause transfersynaptic in global feature and function change, make hippocampal CA1area neuronsin the discharge frequency and discharge type change, thus cause the ability oflearning and memory weaken.As can be seen from the above experiment results, whether long-term (10weeks)or short-term（in an hour） of low intensity electromagnetic radiation, can affectlearning and memory function. But what the molecular biology of these effects andother substances active ingredients and electrophysiological activity change excepthippocampus, also requires us to further research and discussion. We willfundamentally understand the theoretical mechanism of the electromagnetic radiationeffect on learning and memory. What’s more, how to use this theory to work out some effective prevention and control measures, even develop some anti-radiation drugs, isour next major challenge.