Clinical Analysis Of Female Gonadal Hypofunction And Relationship Between Gene Polymorphism Of PPARγ And Diseases Associated With Obesity

Objective To analyze clinical features of childrennbirthing age female gonadalhypofunction. Method Collected the clinical data from60female in child bearing age inthe Department of Endocrinology of Affiliated Hospital of Inner Mongolia MedicalUniversity from May,2011to April，2012retrospectively. Result Among the60patients,there were PCOS of33cases, accounting for55.0%; POF of10cases(16.7%);primaryhypothyroidism(including subclinical hypothyroidism) of6cases(10.0%);prolactinoma of5cases(8.3%);and other6cases.27cases of PCOS were overweight，accounted for81.8%.In10POF patients, FSH level was40-129.3U/L, the average was83.4±23.3U/L, E22.1-52.2pmol/L,26.0±16.2pmol/L on the average. Primary hypothyroidism with TSH>10mIU/L,with T4、FT4decreased， subclinical hypothyroidism TSH>5mIU/L, the average was12.8±12.0mIU/L; In5patients with prolactinoma, PRL level was89.5-126.9ng/ml，theaverage was105.3±12.6ng/ml. Conclusion Common successive causes for childrenbirthingage female gonadal hypofunction were including PCOS, POF, primary hypothyroidism(including subclinical hypothyroidism)， prolactinoma. Therefore, most importantly, thefemale gonadal hypofunction patients should be taken endocrinological examinationsroutinely so that diagnosis can be clarified as soon as possible. Objective Investigate the relationship between PPARγpolymorphism and FemalePolycystic Ovarian Syndrome or male Metabolism Syndrome, and make it clear of therelationship between BMI, blood pressure, plasma lipids or other clinical features and thesyndromes mentioned above. Methods Collect the cases of obesity with Polycystic OvarianSyndrome(67cases aging from18to50years old)from gynecology-endocrine department ofInner Mongolian Women and Kids Health Care Hospital, and obesity with MetabolismSyndrome(84cases aging from18to60years old) from the endocrine department ofAffiliated Hospital of Inner Mongolian Medical University from May,2011to September,2012. General and clinical materials and blood samples are collected in above groups andother two age-matched controls and simple obesity groups. Female group are divided tocontrol(female I),obesity group(female II) and PCOS(female III),male group are divided tocontrol(male I),obesity group(male II) and MS(male III).Gene polymorphism are checked byPCR-RFLP, and proved by gene sequencing. Results1.Group II and III in female havehigher plasma insulin level than group I，the level of T and LH/FSH ratio in group III arehigher than normal（P＜0.05）. Group II in male have higher SBP、DBP、T than group I,HDL-C lower than I, Group III have higher SBP、DBP、TG、FPG than group II（P＜0.05）.2.Genotype frequency distribute of PPARγC161T polymorphism differently among threegroups in female and male（P＜0.05）;T allele frequency in group I、II、III in female are46.4%、20.4%、27.3%，there is statistical significance between group I and II,I and III（P＜0.05），there′s no statistical significance between group II and III（P＞0.05）；T allelefrequency in group I、II、III in male are38.2%、22.2%、68.1%，there is statistical significancebetween group I and II,I and III,II and III（P＜0.05）.3. Genotype frequency distribute ofPPARγPro12Ala polymorphism differently among three groups in female and male（P＜0.05）;A allele frequency in group I、II、III in female are35.6%、60.5%、82.5%，there isstatistical significance between group I and II, II and III, I and III（P＜0.05）; A allele frequency in group I、II、III in male are37%、69.8%、86.2%，there is statistical significancebetween group I and II, II and III, I and III（P＜0.05）.4. Genotype frequency distribute ofPPARγC1431T polymorphism differently among three groups in female and male（P＜0.05）;T allele frequency in group I、II、III in female are82.4%、60%、67.7%，there isstatistical significance between group I and II,I and III（P＜0.05），there′s no statisticalsignificance between group II and II（IP＞0.05）；T allele frequency in group I、II、III in maleare83.7%、62.5%、77.6%，there is statistical significance between group I and II,II and III（P＜0.05）, there′s no statistical significance between group I and III（P＞0.05）.Conclusions1. Simple obesity could accompany higher blood pressure in normal reach; Tlevel and LH/FSH ratio step up in PCOS.2. PPARγC161T polymorphism T allele may beprotective gene in obesity.3.PPARγPro12Ala polymorphism A allele may be the common riskfactor in obesity, PCOS and MS.4. PPARγC1431T polymorphism T allele may be theprotective factor in obesity, PCOS and MS, but the contribution rate to obesity, PCOS and MSis undeterminable.