Single Nucleotide Polymerase In CDH23, PMCA2Gene In Workers Exposed To Industrial Noise

ObjectiveTo investigate the association of cadherin and Plasma membrane calcium ATPaseisoform2single nucleotide polymorphisms with the noise induced hearing loss (NIHL)among the occupational noise exposed workers. If the susceptible genes were identified, thesusceptible individuals could be screen out before exposure to the noise environment,achieve the primary prevention goal.Methods194workers exposed to occupational noise were as the subjects in the epidemiologystudy. According to the result of audiometry, they were divided into two groups: the NIHLgroup and the normal group. Genomic DNA was extracted from whole blood usingstandard procedures of TaKaRa kit. Gene-specific primers for polymerase chain reactionwere designed respectively using Primer Premier5.0software. The genotypes of CDH23genes (rs1227049, rs1227051, rs3802711, Exon7) and PMCA2-rs2289274were deteminedby PCR-RFLP. The genotypes of PMCA2-rs6790640gene were detemined byASA-PCR.data were analyzed with SAS9.0software. Allele and genotype in differentcharacteristic population were compared with chiq-square test. Multiple logistic regressionwas used to calculate the adjusted OR and the95%CI for NIHL.ResultsAccording to the result of audiometry, they were divided into two groups: the NIHLgroup(n=94) and the normal group(n=100). Balance comparison of two group found that education level, noise exposure, workage, cumulative noise exposure had no statisticallydifference, P>0.05; Sex, age had significant differences,P<0.05.The T allele of CDH23-rs3802711is a protective factor of NIHL (OR=0.61,95%CI=0.41~0.92). The allele of CDH23-Exon7is a risk factor of NIHL (OR=4.65,95%CI=2.90~7.44).The G allele of PMCA2-rs12289274is a risk factor of NIHL (OR=2.274,95%CI=1.47~3.53). The T allele of PMCA2-rs6790640is a risk factor of NIHL (OR=1.69,95%CI=1.12~2.53).After adjust for age, sex, cumulative noise exposure (CNE) with multiple logisticregression analysis, the risk of CDH23-rs3802711CC genotype was found significantlyincreased than that of the TT genotype, the OR value of which was0.18,(95%confidenceinterval0.06~049); The risk of CDH23-Exon7GG genotype was found significantlyincreased than that of the AA genotype, the OR value of which was15.87,(95%confidenceinterval3.91~64.45); The risk of PMCA2-rs2289274AG genotype was found significantlyincreased than that of the GG genotype, the OR value of which was13.60,(95%confidenceinterval6.09~30.61).Conclusions1It was suggested that the single nucleotide polymorphism of CDH23-rs1227049andCDH23-1227051might not be a susceptible factor for noise-induced hearing loss.2CDH23-rs3802711T allele is a protective factor of NIHL; CDH23-Exon7A andPMCA2-rs12289274G are risk factors of NIHL. CDH23-rs3802711CT genotype is aprotective factor of NIHL, CDH23-Exon7GG genotype and PMCA2-rs12289274AGgenotype are risk factors of NIHL.5In a preliminary conclusion, CDH23-rs3802711,Exon7and PMCA2-rs6790640could be used as molecular markers of NIHL.