Research On Control Release Of SiRNA Mediatedby Mesoporous Sillicon Nanoparticles

Mesoporous silicon nanomaterials(MSN) due to the huge specific surface, poresize adjustable, easy to modify, higher biocompatibility characteristic, more and morereceived the attention of people, Especially, as a genetic drug carrier has theincomparable superiority than other carriers, In this paper, this expected to make thenanoparticles carry siRNA and medicine to safely and efficiently transfection thecells.end achieve controlled release.first synthetic MSN, which through calcined, amination modify, carboxyl modify,modified nanoparticles pass a series of characterization, end synthetic HA of Capstructure. detected by SEM, TEM, Zeta potential, XRD, FTIR. The characterizationresults show that the nanoparticle is a kind of homogeneously dispersed spherical withaverage particle diameter about160nm, pore diameter about2.6nm. After modifyelectrical charged but the other characterizations not changed.Different modified MSN used for rhodamineB，CPFX and siRNA adsorption,release and protect The result suggest that different modified MSN adsorption quantityhave significantly different, N-MSN adsorption quantity is the biggest. Under differentconditions to release, in deionized water X-MSN, D-MSN, N-MSN, S-MSN to release aquantity of rhodamine B is28.7mg,16.3mg/g,43.7mg/g/g,28.9mg/g; in deionizedwater X-MSN, D-MSN, N-MSN, S-MSN of CPFX to release a quantity to25.92mg/g,15.68mg/g,39.04mg/g,20.68mg/g,alkaline conditions to release a quantity is higher.X-MSN,D-MSN, N-MSN, S-MSN, adsorption of siRNA70.82mg/g,12.9mg/g,88.8mg/g,14.5mg/g, respectively. at the different of pH release of siRNA indicate thatalkaline conditions conducive to release siRNA, especially N-MSN about58.7mg/g.Agarose gel electrophoresis indicate that MSN can against nuclease degradation andprotect siRNA.the HA-capped MSN to release a quantity to different of siRNA. acetate buffer(pH4.5) that contained Hyal-1(150U/mL) relaese of siRNA61.4mg/g, acetate buffer(pH4.5) without Hyal-1and PBS buffer(pH7.4) have similar release about25.6mg/g,the enzyme was denatured by heating, relaese about23.7mg/g.Final, different modified nanoparticles transfer cell of GFP-MCF7, through theconfocal fluorescence microscope and flow cytometry observation intake condition andcorresponding gene silence efficiency In addition, determination of nanoparticles for thecell toxicity, the result show that MSN Non-toxic and safety, through the aboveexperiments, shows the MSN gets the potential to become an ideal gene vector.