Adenovirus Vector-mediated Gene Transfer In Vivo Vascular Experiments

Part â…  In vivo vascular gene transfer using solublestentPurpose: The possibility of gene transfer into anastomotic arteries in vivo using soluble stent containing Adv5-CMV/LacZ was studied in this experiment. Materials: After being soaked in high concentration of glucose solution containing Adv₅-CMV (control group) or Adv₅-CMV/LacZ (treatment group) for 30 minutes. Soluble stents were inserted into the lumina of cut rat carotid arteries and end-to-end anastomoses of cut rat carotid were performed with standard microvaspular surgical technique. 16 rats were killed after two weeks, the segments of anastomotic carotid arteries were prepared for β -galactosidase activity and histochemical staining. Results' In control group, the anastomotic arteries did not have detectable level of β -galactosidase expression. In treatment group, the amount of β -galactosidase expression was 9.80×,10^-3u/gtissue. Microscopicexamination of histochemically stained arteries demonstrated gene transfered not only to endothelium cells but also to smooth muscle cells and all anastomotic arteries were transfered in treatent group, but none of arteries revealed blue in control group. Conclusion: The results of β -galactosidase activity and...