Comparative Study On Cinnamaldehyde And Citral Against HL-60Cell Growth Effect And Mechanism

As we all know, leukemia is a hematopoietic malignancies, for the domestic one ofthe top ten high-issued tumor disease, and the incidence rate showed an increasing trendyear by year. Therefore depth research on the pathogenesis of leukemia and developmentof new, high efficiency, low toxicity, and inexpensive drug, is not only related toprofessional researchers engaged in the most urgent task, and is related to the child andadolescent health event and family happiness event.Western treatment of leukemia commonly used chemotherapy, radiation therapy andblood stem cell transplantation, but the chemotherapeutic is major. Chemotherapy orradiation therapy to patients with pain, and transfusion or exchange transfusion but alsothere are many security risks. In contrast, the conservative Chinese medicine treatment ofleukemia has unique advantages, not only rich in resources, unique effect, and drug sideeffects, safety problems is small, many channels simultaneously, if there are long-term useof Chinese medicine to protect and promote the function of bone marrow hematopoieticsystem increased role. Currently, the use of pure Chinese medicine treatment of leukemiaserves as superior, it is anti-leukemia medicine to provide new ideas and methods havebecome an inevitable trend.And citral against HL-60cell disease compared to the subjectthat is not just for cinnamaldehyde, has good selectivity, with less effect, low toxicity,showing the pre-clinical studies and and developing prospects, but also this aldehydes onlyhas trans-cinnamaldehyde monomer, provided the better conditions for the synthesis andpreliminary study on molecular mechanism on the cells in human promyelocytic leukemiacell line HL-60.The using methods in this paper:（1） Benzene formaldehyde and acetaldehyde in the acidic conditions, synthesis oftrans-cinnamic aldehyde.（2） Stained by trypan blue exclusion method HL-60cell growth curve,Tacking twoaldehyde on the cell line HL-60function of process.（3） Thiazolyl blue (MTT) assay cinnamaldehyde and citral different doses and time on the HL-60cell growth;（4） Application of AO/EB staining, morphological observation by scanning electronmicroscopy, transmission electron microscopy method to observe the morpho-logical changes of cell morphology,to reveal the two aldehyde on the cell lineHL-60of a single standard rules of changes.（5） Flow cytometry testing cinnamaldehyde and citral on apoptosis rate of HL-60cells to the determination of mitochondrial membrane potential, intracellular ROSproduction volume, the change of metabolism on the role of the two aldehyde on thecell line HL-60.Results: Cinnamaldehyde and citral were inhibited HL-60cell growth and inducedapoptosis in biological effects, while the former than the latter good selectivity, lowconcentrations of cinnamaldehyde can be found through the membrane into the celldamage that increased mitochondrial oxygen consumption rate; can induce apoptosis,inhibit the growth of HL-60cells.By the determination ROS, Although the latter process ofapoptosis is similar, the former is presented through the cavity of the cells scattered,leading to nuclear apoptosis is a contraction, followed by the same drug dose is the amountof apoptosis, but the former is less than the latter, excluding cells of inflammation，Toachieve from a single cell and subcellular level in real time, in situ, without disturbing thestate of trans-cinnamic aldehyde interpreting anti-HL-60cells, the mechanism for its initialclinical application of theoretical basis and technical parameters.