Build Magnesuim-substituted Nanohydroxyapatite Coating On Sandblasted-dual Acid Etched Titanium Surface And Evaluate Its Biology

Objective:In this research, the purpose is to study the possibility of the porous Titanium with electrochemically deposited Magnesuim-substituted nanohydroxyapatite and Nanohydroxyapatite coatings and evaluate their biological activities.Method:Magnesuim-substituted nanohydroxyapatite and Nanohydroxyapatite coating were deposited on sandblasted-dual acid etched titanium surface by electrochemically deposition. The surface topography and the contact angles of the material were characterized by Field-emission Scanning Electron Microscopy (FSEM) and SL200B contact angle measuring apparatus respectively. The mouse bone marrow stromal cells were then cultured on the porous Titanium with Magnesuim-substituted nanohydroxyapatite coating, porous Titanium with Nanohydroxyapatite and porous Titanium surface and testing for the cell proliferation by MTT assay. The effects of the alkaline phosphatase activities as well as the amount of the osteocalcin in the culture media on cell differentiation were also studied and evaluated.Results:The mouse bone marrow stromal cells are able to proliferate and grow well on the modified layers. All the tested surface, sandblasted-dual acid etched Titanium with Magnesuim-substituted nanohydroxyapatite coating, sandblasted-dual acid etched Titanium with nanohydroxyapatite and sandblasted-dual acid etched Titanium showed good biological activities.Conclusion:Magnesuim-substituted nanohydroxyapatite active layers that manufactured by electrochemically deposit method showed good biocompatibilities and abilities to promote both proliferation and differentiation of bone marrow stromal cells on the materials surface. Titanium with this treatment is believed as the implantable materials in the future applications.