| Objective Chronic kidney failure (CRF) is a serious clinical symptoms occured in a variety of end-stage chronic kidney disease, medical care for the public health issue is a major challenge, manifested in decreased kidney detoxification, water and salt metabolism, endocrine disorders, with a total of short or long trm survival of patients have a significant impact. The progression of CRF is characterized by the development of glomerular and tubular lesions about which multiple factors can be involved. As complex pathology and pathogenesis of chronic kidney disease and its pathogenesis is unclear at the moment, there are no effective treatments to slow and treatment of chronic kidney disease. In fact, eventually leading to the CRF is no doubt.Moreover, tubular interstitial fibrosis is a common pathological mechanism of various chronic kidney disease developed to renal failure, and normal functional tubular epithelial cells in kidney fibrosis significantly were reduced or disappeared, partly due to damage factors such as uremic toxin serum, on the other hand, because of trans-differentiation. Therefore, the primary culture of rat renal tubular epithelial cells (RTECs) in vitro would be valuable in a variety of studies on the factors and mechanisms responsible for diseases and disorders of the kidney. Primary renal tubular epithelial cells have cultured successful using Direct screen method, sieve conjugating enzyme digestion.Chitin-oligosaccharides and Chitosan-oligosaccharides (COS), is a type of low degree polymer and water-soluble of amino-oligosaccharide with unique biological and physiological activity. Studies have shown that chitin-oligosaccharides and COS have many outstanding effects, and COS has achieved good results of treatment of acute renal failure. Chitin-oligosaccharides and COS has become a hot topic in the field of carbohydrate research. Effects and mechanism of oligomeric amino-oligosaccharide on renal damage induced by adenine is not clear. This study used chitin-oligosaccharides and Chitosan-oligosaccharides investigated their effects on the factors influencing renal function and renal histopathology for adenine-induced renal lesions in vivo and in vitro.Methods Study for Wistar rats and research the protective effects of the oligomeric aminooligosaccharide on chronic renal failure rats, established by gavage with adenine carboxymethylcellulose suspension for4weeks. The levels of serum creatinine (SCR), urea nitrogen (SUN) were significant higher in the adenine-induced rats than those in the control group, indicating that the rat chronic renal failure model was made successfully. CRF model group were randomly divided into model control group, Naoduqing group, COS and Chitin-oligosaccharides High\Medium\Low groups, were treated for4weeks. The general living conditions of the rats were observed and recorded, kidney weight coefficient were calculated, blood samples were collected by abdominal aortic with lancets and kidneys tissue homogenates were made of each group at different times of treatment, in order to determine the levels of SCr and SUN, and enzymatic activities of Total Superoxide Dismutase (T-SOD), glutathione peroxidase (GSH-PX). A portion of kidney tissue was fixed, stained with hematoxylin and eosin for routine histological examination. The experiment in vitro, primary renal tubular epithelial cells were cultured using mesh sieve enzyme digestion method and the growth effects of COS on RTECs were studied using MTT assay.Results In vivo, COS and chitin-oligosaccharides have effect on reducing the SUN and SCR levels and elevating the activities/levels of T-SOD and GSH-PX in kidney compared to model group. In COS low dose group and chitin-oligosaccharides high dose group, the levels of SCr and BUN are100%/63.75%and63.75%/52.93%lower, while the T-SOD and GSH-Px activities of renal tissue are63.36%/62.41%and82.16%/55.90%higher than those in the model group respectively and better than the Niaoduqing group. Based on pathological findings in this study, adenine impaire renal tissue, and lead to the glomerular and tubular lesions. COS and chitin-oligosaccharides can alleviate the rat kidney tissue of chronic renal failure in renal parenchymal damage.In vitro, MTT-test results analyzed, COS in125-500μg/mL, have different degrees of promoting growth effect on renal tubular epithelial cells and the effect is most significant at250.μg/ml.Conclusion In vivo, the results suggeste that the COS and chitin-oligosaccharides have protective effects on chronic rennal failure model induced rats by ademine. In vitro, COS promotes the growth of RTECs, relevant to improving renal function. |
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