| ObjectiveTo investigate the effects of enteral feeing temperature on rats induced by gastricischemia reperfusion injury (GI-RI)and explore it’s probably mechanism.MethodsA total of forty-eight male Sprague-Dawley rats were randomly divided into four groupsaccording to enteral feeding temperature:10℃,24℃,32℃and40℃(n=12per group). Gastricischemia-reperfusion injury was induced in rats by clamping the celiac artery for30minutesand then reperfusing. At one hour after the reperfusion,two rats from every group wererandomly selected to sacrifice. The other rats received enteral feeding at two hours after thereperfusion, six times per day. We observed the subsistence conditions, gastrointestinalcomplications, body weight and body temperature (rectal temperature) of rats. Whenforty-eight hours later after the reperfusion，gastric mucosal injury index was counted grossly,pathological score of gastric mucosal injury was evaluated using a light microscope. Thelevel of TNF-α，MDA，SOD in gastric mucosal and TNF-α, MPO in plasma weremeasured.Results(1) A large amount of dot, funicular, schistose erosions and petechias on gastric mucosalin rat were observed grossly and gastric mucosal layer lesions were observed by lightmicroscope at one hour after gastric ischemia reperfusion.(2) Comparing other groups, the values of gastric mucosal injury index, pathologicalscore, TNF-α, MPO, MDA in10℃group were decreased. Meanwhile, the SOD activitywas increased. The gastric mucosal injury, pathological score, the positive expression ofTNF-α, MPO and MDA in gastric mucosal were positively correlated with enteral feedingtemperature(P＜0.01), SOD was negatively correlated with enteral feeding temperature(P＜ 0.01). However, there was no statistically different among four groups about the level ofplasma TNF-α.(3) There was a significant difference in diarrhea rate(P＜0.01), the number of diarrhearats in10℃group was more than the other groups, however, no diarrhea was found in40℃group. The enteral feeding temperature did not significantly alter gastric residual andflatulence(P＞0.05). The weight of rats in10℃group was the lowest after the surgery. Butthe rectal temperature did not associated with the enteral feeding temperature(P＞0.05).Conclusions(1) These results demonstrate that enteral feeding temperatures of10℃exhibits agastroprotective effect against acute gastric mucosal lesions in rats induced byischemia-reperfusion, which probably involves suppressing the early inflammatory response,restraining neutrophils gathered, promoting the balance of peroxidation and antioxidation byhypothermia. Enteral feeding temperatures of24℃and32℃show better gastroprotectiveeffect than40℃.(2) The enteral feeding temperatures of24℃、32℃and40℃show less side-effect than10℃, such as the incidence of diarrhea and postoperative nutritional depletion.(3) enteral feeding temperature from24℃to32℃is more suitable for the rats inducedby GI-RI. |
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