The Relationship Of Intestinal Flora And Non-alcoholic Fatty Liver Disease

Objective:To analyze the quantitative differences of Eubacterium rectale, Bacteroides thetaiotaomicron,Lactobacillus and bifidobacterium in non-alcoholic fatty liver patients and the control group (normal metabolism).To explore the role of intestinal flora in the occurrence and development of non-alcoholic fatty liver disease.Methods:In our study there were150subjects with NAFLD were selected as research objects,and60cases of normal metabolism without NAFLD were selected as the control group.Fasting blood glucose,2hour plasmer glucose,lipid spectrum and liver enzyme were detected. Extracted the total DNA of intestinal flora, the expression level of DNA of target bacteria were detected by relative quantification of Real Time PCR.Results;1.We found that BMI in NAFLD patients were significantly higher than the control group (p=0.00).2. FPG,2hPG, HbA1c, TC,TG and LDL-C in NAFLD patients were significantly higher than the control group (P<0.05),but there was no difference in HDL-C(P>0.05).3. ALT and AST in NAFLD group were significantly higher than the control group(P<0.05). ALP and GGT were higher than the control group,but there was no significant differences (P>0.05).4. The quantity of Eubacterium rectale and Lactobacillus in NAFLD patients had a statistically significant increase compared to the control group(P=0.007, P=0.00), the quantity of Bacteroides thetaiotaomicron and Bifidobacterium were significantly decreased (P=0.022, P=0.00).5. Compared to the NAFLD patients with normal glucose tolerance,the quantity of Eubacterium rectale and Lactobacillus in NAFLD patients with type2diabetes patients were significantly increased (P=0.00,P=0.006), and Bacteroides thetaiotaomicron was significantly decreased (P=0.002),but there was no different in the quantity of Bifidobacterium(p>0.05).6. Compared to the control group,the quantity of Eubacterium rectale and Lactobacillus in NAFLD with type2diabetes patients had a statistically significant increase (P=0.00),Bacteroides thetaiotaomicron and Bifidobacterium were significantly decreased (P=0.00).7.Compared to the the control group, the quantity of Bifidobacterium in NAFLD with normal glucose tolerance patients was significantly decreased (P=0.00),but the quantity of Eubacterium rectale, Bacteroides thetaiotaomicron and Lactobacillus had no significant difference (p>0.05).Conclusion:1. BMI in NAFLD patients was significantly increased compared to the the control group, demonstrating that NAFLD patients accompanied by overweight or obesity.FPG,2hPG,HbA1c,TC,TG and LDL-C were significantly increased, suggesting that NAFLD patients accompanied by blood glucose and lipid metabolic disorder.ALT and AST were significantly increased, indicating that non-alcoholic fatty liver patients presence of abnormal liver function.2.The quantity of Eubacterium rectale and Lactobacillus in NAFLD patients were significantly increased compared to the control group,and Bacteroides thetaiotaomicron,Bifidobacterium were significantly decreased. Indicating that patients with NAFLD may exist structural imbalance of intestinal flora.3. Compared to the NAFLD with normal glucose tolerance and the control group, the quantity of Eubacterium rectale and Lactobacillus in NAFLD with type2diabetes patients were significantly increased,the quantity of Bacteroides thetaiotaomicron was significantly decreased,but in the NAFLD with normal glucose tolerance and the control group, there was no significant difference in the quantity of this three kinds of target bacteria. Prompting in NAFLD when glucose metabolic impaired,may be due to the factors of metabolic abnormalities agglomeration, then leading to the structural change of the intestinal flora, breaking the balance of the gut-liver axis, thus promoting the subsequent development of the disease.4. Compared to the control group,the quantity of Bifidobacteria in NAFLD with type2diabetes or normal glucose tolerance patients were significantly decreased, showing that the quantity of Bifidobacteria probably has been reduced at the stage of normal glucose metabolism in NAFLD patients, it may played a promotion role in the pathogenesis in the early stages of NAFLD or type2diabetes.Indicating added Bifidobacteria may has a protective effect on type2diabetes and NAFLD patients.