The Relevance Research Of Viral Load And T Lymphocyte Subsets In HIV/HBV/HCV Coinfection

Objective Researching the correlation among viral loads of the patients living with HIV/AIDS, hepatitis b virus (HBV) and hepatitis c virus (HCV), and the relationship with T lymphocyte subsets. To explore Whether or not HIV/HBV/HCV coinfection will lead to three kinds of virus biological characteristics and its changes in the level of T lymphocyte subsets count, thus caused the cell immune regulation mechanism of complication and diversification, for effective diagnosis and treatment and control hepatitis B virus, hepatitis C virus, HIV/AIDS to provide certain theoretical basis.Methods By the fluorescent quantitative PCR and Flow cytometry technology (Flow cytomertry, FCM) respectively to detect HIV/HBV/HCV viral load and T lymphocyte subsets count of the631cases of patients with HIV/AIDS who come from the outpatient and inpatient in our hospital,the third people’s hospital and Dehong prefecture, using a variety of mathematical model analysis to discuss the correlations between viral load and T lymphocyte subsets count in HIV/HBV/HCV coinfection.Results1. Study a total of631people, male474, female157, age11-82(average age40.70±11.19years), including424sexual contact with infected people, intravenous drug use infected191people, other ways patients16people.2.631cases of the research object in the pure people infected with HIV,277people, accounting for43.90%(277/631); HIV/HBV coinfection infected48people, accounting for7.61%(48/631); HIV/HCV coinfection infected186people, accounting for29.48%(186/631); HIV/HBV/HCV coinfection patients24people, accounting for3.80%(24/631).3. The difference between HIV/HBV coinfection and HIV/HCV coinfection group on the liver function injury caused no statistical significance (P>0.05); the differences between HIV/HCV coinfection, HIV/HBV/HCV coinfection and HIV infection alone group triple on the liver function injury caused with a statistical significance (P<0.05).4. Sexual contact with infected people in HBsAg positive rate was12.5%, positive rate of anti HCV-IgG (15.57%); Intravenous drug use in people infected with HBsAg positive rate was9.42%, positive rate of anti HCV-IgG (73.82%); Positive rate of anti HCV-IgG, intravenous drug use infection group was obviously higher than sexual contact infection group (P<0.01), while HBsAg positive rate, infections of intravenous drug use and sexual contact group there was no statistically significant difference (P>0.05).5. HIV RNA (log10cp/ml), CD4, CD8, CD4/CD8, the difference was statistically significant between Pure HIV infection and HIV/HBV coinfection group(P<0.05), and in the HIV/HBV coinfection HIV RNA (log10cp/ml) with HBV DNA (log10cp/ml) had positive correlation (r=0.450, P<0.05).6. Between HIV infection alone group and HIV/HCV coinfection, HIV RNA (log10cp/ml), CD4and CD8difference was statistically significant (P<0.05), and in the HIV/HCV coinfection group HIV RNA (log10cp/ml) with HCV RNA (log10cp/ml) has the positive correlation (r=0.365,P<0.05).7. The HIV RNA(log10cp/ml), CD4and CD4/CD8differences had statistical significance between HIV/HBV coinfection and HIV/HCV coinfection group(P<0.05).8. In HIV/HBV/HCV coinfection there was the positive correlation between HBV DNA (log10cp/ml) and HCV RNA (log10cp/ml)(r=0.708, P<0.05), CD4, CD8and CD4/CD8with HBV DNA (log10cp/ml), there is negative correlation (r=-0.695、-0.533.-0.524, P<0.05), CD3with HBV DNA (log10cp/ml) no correlation (P>0.05); The CD4, CD8, CD3and CD4/CD8with HCV RNA (log10cp/ml) had no correlation (P>0.05).Conclusion①HIV/HBV/HCV coinfection occurred more often in men, through intravenous drug use and HIV/HCV coinfection, occurred more often in HIV/HBV coinfection, two transmission way there is no difference.②HBV and HCV may have promoting HIV RNA replication effect, and the role of HBV in HIV RNA replication, and then for HIV RNA replication quantity growth accelerated the damage of T lymphocyte subsets.③Total infection with HBV and HCV in T lymphocyte subgroup than HIV infection alone are more likely to be damaged, and HIV/HBV/HCV triple infection when the T lymphocyte subgroup damage further than when HIV/HCV coinfection.④HIV/other diseases (AIDS related opportunistic infections and tumors), may also lead to increased HIV RNA replication and T lymphocyte subgroup of further damage, and the total infection fungal infection after damage to the airframe immunity levels than altogether more serious after infected with TB, with infection after must pay attention to the timely processing.⑤HIV/HBV/HCV infection of triple could promoted the HCV RNA replication of HBV, may also inhibit HCV RNA replication, but there is the possibility of promoting effect.