The Proteins Differential Expression In The Blood Serum Of Systemic Lupus Erythematosus

[Objective] Application of surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) to identify proteins differentially expressed in the blood serum from patients with systemic lupus erythematosus (SLE) active group stable group and normal control group. we hope to search different proteins in whole lever and uncover novel biomarkers.To explore the pathogenesis and some effective biomarkers of SLE.[Methods] SELDI-TOF-MS and protein chips weak cation exchange (CM10chip) were used to detect the protein mass spectra and screen out the differential proteins in the sera of40patients with SLE active group and40patients with SLE stable group and40normal control group.The protein mass spectra and the differential proteins in the sera were further studied by statistical analysis and Hierarchical Cluster Method and principal component analysis.To identified the protein by bioinformatics database.[Results]1. There were significantly different expression of53proteins peaks between the SLE active group and SLE stable group (P<0.05).21of them were up-regulated in the SLE active group,32of them were significantly down-regulated.2.There were significantly different expression of22proteins peaks between the SLE stable group and normal control group (p<0.05).15of them were up-regulated in the SLE stable group,7of them were significantly down-regulated.3.There were significantly different expression of44proteins peaks between the SLE active group and normal control group (P<0.05)).17of them were up-regulated in the SLE active group,27of them were significantly down-regulated.4. Identidied the protein by bioinformatics database, the expression of hepcidin increased in SLE active group than that of SLE stable group/ normal control group. The expression of neurosecretory protein vgf frag and amyloid beta a4protein increased in SLE active group than that of SLE stable group. The expression of neutrophil defensin1and neutrophil defensin3decreased in SLE active group than that of SLE stable group. The expression of plasma protease c1inhibitor frag was low in SLE active group than that of SLE stable group/normal control group. The expression of complement C3frag was low in SLE active group than that of normal control group. The expression of alpha-1-antichymotrypsin decreased in SLE active group than that of SLE stable group. The expression of inter-alpha-trypsin inhibitor heavy chain h4frag decreased in SLE stable group than that of normal control group.[Conclisions]1. There were some different proteins expresssion between SLE group and normal control group sera.2.Hepcidin maybe associated with SLE renal flare, and hope to be biomarker of SLE flare.3.Neutrophil defensin1and neutrophil defensin3have function of chemotaxis, regulatory effect on phagocytosis, cytotoxicity, and complement activation.4. Complement C3maybe has significant clinical value in diagnosis of SLE.5.The significance and function of amyloid beta a4protein, alpha-1-antichymotrypsin, fibrinogen alpha chain frag, inter-alpha-trypsin inhibitor heavy chain h4frag, plasma protease c1inhibitor frag and neurosecretory protein vgf frag need to further study.