The Protein Mass Spectrometry High-throughput Analysis Platforms Established And Applied In Digestive Cancer

Because of the multi-factorial nature of cancer, it is very clearly that the combination of several markers is necessary to effectively detect and diagnose cancer. Digestive cancer is the most lethal malignancy in china. The death rate of esophageal, gastric and colorectal cancer is the highest in the common cancers. The current serum markers for digestive cancer have a poor diagnostic value. Therefore, it is urgent to find sensitive, specific, and convenient new biomarkers.Protein but not nuclear acid is the material executant and embodiment of life. So, the studies on proteomics are preferred to approach to the pathogenesis of breast cancer and screen sensitive and specific biomarkers. A novel technology, surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) carved out such a new path to proteomics research on cancer. It can directly obtain high-throughput protein profiling from clinical samples with high sensitivity and this is the main advantage of this technology. But the reproducibility of the experiment is relatively deficiency.With the rapid development of life science, large quantities of data were obtained from proteomics fields. It is almost impossible to analysis so much data only by traditional mathematic and statistic methods. So bioinformatics is applied to collect, deposit and analysis the data and screen the useful information. The high-throughput data analysis software will improve the experiment efficiency, and improve the reproducibility of the protein peaks of mass spectrometry.Identify the candidate cancer related biomarker is another crucial step of biomarker studies. The further studies of tumorigenesis can be continued.Three main parts compose this study. First, establishing the standard protocol of serum protein mass spectrometry to overcome the deficiency of reproducibility. Second, developing the high-through protein mass spectrometry data analysis platforms. Third, applied the protein mass spectrometry high-throughput analysis platforms in digestive cancer and identify the related biomarkers.