Effect Of Curcumin On Expression Of P-ERK1/2and P-P38Mapkingastriccarcinomasgc-7901Cells

Objective By in vitro cultured human gastric carcinoma SGC-7901Cells approach to different concentrations of curcumin in SGC-7901, see if can inhibit cell proliferation, and its effect on MAPKs signaling pathway P-ERK1/2, p-p38MAPK expression and other aspects, to investigate the value of pro-apoptotic effects.Method:The conventional culture of human gastric carcinoma SGC-7901Cells in general. Inhibition of different concentrations of curcumin (10,20,30,40,50μ mol/L) in SGC-7901cells treated with24h,48h,72h, proliferation of cells was determined by MTT method; curcumin at different concentrations were determined by Western-Blotting method (10,20,30,40,50μmol/L) respectively in SGC-7901cells at different time (15,30,60min), changes of P-ERK1/2, p-p38MAPK expression in SGC-7901cells.Results:1MTT results showed that different concentrations of curcumin significantly inhibitory effect on human gastric carcinoma SGC-7901Cells cells proliferation, and were time and dose dependent (P<0.05).2Curcumin reduced p-ERK1/2expression, inhibition of human gastric cancer cell line SGC-7901in the p-ERK1/2signaling pathway.3Curcumin can increase the expression of p-p38MAPK, activation of SGC-7901in the p-p38MAPK signal transduction pathway.Conclusion:Curcumin can inhibit human gastric cancer cell line SGC-7901cell proliferation.Curcumin could down-regulate the expression of p-ERK1/2in SGC-7901cells of human gastric cancer cell line, the inhibition of P-ERK1/2signal transduction pathway; curcumin can increase the expression of P-p38MAPK in gastric carcinoma SGC-7901Cells, activation of P-p38MAPK signal transduction pathway.