| Objective:to discuss the relationship between gastroesophageal reflux and bronchial asthma as well as the possible pathogenetic mechanism.Methods:Choose60male mice to divide into4groups randomly. Group A B C and D. A (normal control group), B (NS control group).C (asthma control group). D (bronchial asthma control and gastroesophageal reflux group). A is the normal control group. Take the intraperitoneal infection, aerosol inhalation and gavage with NS to the mice in Group B. Make the mice of C and D supersensitive by using ovalbumin, OVA adding aluminium hydroxide, take the aerosol inhalation from the22nd day, using2%OVA physiological saline solution30minutes once a day, lasting7days. While to D, take the gavage to the mice by using hydrochloric acid and pepsin, lasting21days.For the asthma control group, firstly, attack the mice by giving the ovalbumin, then take the aerosol inhalation again, using2%OVA physiological saline solution30minutes after7days, in order to cause the asthma. For the bronchial asthma control and gastroesophageal reflux group, when attacking the mice by giving the ovalbumin was over, a week later, take the aerosol inhalation again us ing OVA (10mg/ml) physiological saline solution30minutes in all and complete the last gavage to the mice. For the NS control group, take the aerosol inhalation using OVA physiological saline solution, bronchus stimulate the mice within24hours when the last gavage was over. Now, monitor the ratio airway resistance ratio of the four groups of the mice, watch the change of the bronchial hyperresponsiveness. After finishing pulmonary alveoli douche smear, count the Eosnophils granulocyte, also work out the percent. Test the change of IFN-γ、IL-4as well as Thl/Th2in the splenocyte according to the Flow Cytometry. Conduct the HE dye to the lung tissue and observe the pathological section so as to judge the pathomorphism change.Results:1. After the bronchial provocation test, the airway resistance ratio has no statistics difference (P>0.05) comparaing group B to A, while the ratio has statistics difference (P<0.05) comparaing group C and D to A, in addtion, D ratio is superior to C, has statistics difference (P<0.05).2. For IL-4in four groups, comparaing group B to A has no statistics difference (P>0.05),. while the ratio has statistics difference (P<0.05) comparaing group C and D to A, in addtion, D ratio is higher to C, has statistics difference (P<0.05).3. IFN-γ worthy of the four groups, the ratio has no statistics difference (P>0.05) comparaing group B to A, while the ratio has statistics difference (P <0.05) comparaing group C and D, as well as matching D with C.4. Thl/T.h2ratio in four groups, the ratio has no statistics difference (P>0.05) comparaing group B to A, while the ratio was dramatic decline comparaed D to C, has statistics difference (P<0.05).5. Comparison of the percentage that the amount of EOS in BALF takes up the total cellular score, In A, B groups, we can only find single eosinophils (EOS) in BALF accidentally. While there were a lot of EOS in C, D groups, accounted for a percentage of total cells was significantly higher than the A, B group, the differences were statistically significant (P<0.05). The percentage of D was significantly higher than C, the differences were statistically significant (P<0.05).6. In lung tissue biopsy we can observed in group C, D that bronchial epithelial cells dropped, hyperplasia in parts, basement membrane thickened, submucosal stroma hyperaemia oedema, mainly eosinophils of chronic inflammatory cells infiltration, focal alveolar cavities and alveolar walls became thinner. Bronchial wall in group a and b was priority pseudostratified ciliated columnar epithelium, lumen was regular, no interstitial inflammatory cell infiltration.Conclusion:Gastroesophageal reflux can aggravate the bronchial hyperresponsiveness, IL-4level, the amount of acidophilic cells in the pulmonary alveolar lavage fluid and the pathologic change of the lung tissue, can also reduce IFN-γ level and the ratio of Th1/Th2. |
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