Study On Extraction And Separation Of Betaine From Beet Molasses

Betaine, a valuable alkaloids, has shown a wide range of pharmacological activities. In this paper, the influence of cation exchange resin on adsorption characteristics of betaine, the decolorization of beet molasses and the extraction process of Betaine were investigated in detail. The results showed as following:Firstly,732Cation exchange resin showed a better betaine adsorption against other five resins and its adsorption characteristics demonstrated that the equilibrium adsorption capacity is obtained at0.5h and pH3is effective with the static adsorption. However, different temperatures showed little influence on the betaine adsorption. The dynamic adsorption showed that the betaine adsorption was above80mg/g and the most suitable for both adsorption and desorption flow velocity were all0.5mg/mL. Different sample concentrations performed little influence on the adsorption.Subsequently, the effect of activated carbon discoloration, peroxide hydrogen decoloration and resin method on the beet molasses decolorization were studied. While using activated carbon discoloration, the efficient conditions contained5%of activated carbon,50℃of the adsorption temperature, the adsorption time was1.5h and pH was3. In the peroxide hydrogen decoloration, the reaction equilibrium time was1.5h. The best decolorization result was obtained while temperature and pH were50℃and11, respectively. Different temperature and pH performed significant influence on the decolorization. Resin method could combine decoloration and recovery of pigment. The static adsorption showed that the efficient decolorization was obtained with the conditions of5%macroporous resin,25℃, pH9,3.5h of adsorption time. Meanwhile, dynamic adsorption demonstrated that the most suitable for both adsorption and desorptionflow velocity were all0.5mg/mL. Under above conditions, the optimal recover rate of pigment was obtained.Thirdly, a promising approach was developed to extract betaine from beet molasses. The results showed that flow velocity nearly has no effect on the extraction yield by cation exchange resin, while temperature showed the significant influence on it. A satisfactory yield of92.83%and extracting rate of49.53%were obtained at5℃. Furthermore,(CH3)3N’CH2COO-was identified as the structure of betaine.