The Relationship Of Glutamine With Type2Diabetes And Its Mechanism Research

Objective: To study the glutamine(Gln) and the relationship with type2diabetes, and its effect to fasting blood-glucose(FBG)、blood lipids and target bacteria inintestines act on diabetic mice. Methods:①Use HPLC to determine the content ofglutamine in Plasma of Uygur and Kazak ethnic.To determine fasting serum insulin(FINS) by using the radioimmunoassay and TC,TG,HDL-C,LDL-C through automatic biochemical analyzer.②Alloxan-induced diabetes mice, to observe the change ofweight and FBG after giving Gln intervene to model+Gln group and Gln group.The content of C-Peptide and the glutamine content were determined by enzyme-linked immunosorbent assay (ELISA) in the serum of the mice. To determine TC, TG, HDL-C, LDL-C in serum of the mice, by using automatic biochemical analyzer.③DNA was extracted from fecal samples of mice, the level of the bacteroides thetaiotaomicron was determined by Real-time PCR. Result:①The glutamine contentwas significant difference between NGT’s and T2DM in the Uygur(P<0.01), but there was no significant difference between the NGT’s and T2DM in the Kazak；There was significant difference between the Uygur and Kazak normal person (P<0.01), there was significant difference between the Uygur and Kazak, who with type2diabetes mellitus (P<0.05). Glutamine content was negatively correlated with TC(r=-0.4483, P=0.006) in Kazakh people. In Uygur people, glutamine content was positively related with HOMA-IS(r=0.52,P=0.001),but negatively with FBG(r=-0.61,P<0.001), HOMA-IR(r=-0.55,P<0.05),TG(r=-0.5064,P<0.05), LDL-C(r=-0.5621,P=0.008).②The Gln have effect on Gln group and model+Gln group: compared with blankcontrol group, Gln could reduce the weight of Gln group after giving Gln, and there was significant difference(P<0.01), model+Gln group compare with model group,there was significant difference from the4th week(P<0.05); About FBG, there wassignificant difference on week3and4(P<0.05), between model+Gln group and m odel group; About C-peptide compare with model group, model+Gln group and Gln group all have significant difference(P<0.05); There were significant differences(P<0.05)about IRI, compared with model group, in model+Gln group and Gln group; About HOMA-IS, compared with model group, model+Gln group and Gln group all have significant difference(P<0.01). Obtain by correlational analyses, glutamine content was negatively correlated with FBG(r=-0.45,P<0.05), HOMA-IR(r=0.3216,P<0.05), LDL-C(r=-0.5091,P<0.01) and positively correlated with C-peptide(r=0.4584,P<0.05),HOMA-IS(r=0.3216,P<0.05).③Alloxan was able to induce diabetes in35%(7/20) mice for Gln group, but Alloxan was able to induce diabetes in50%(10/20)mice for the control group.④There was not significant change for Colonic pathological section, and model+Gln group compare with model group, there was no significant difference in Bacteroides thetaiotaomicron. Concludes: There was significant difference of the glutamine content between Uygur and Kazak, That may be show glutamine maybe a protective factors to type2diabetes. Gln could reduce theweight and FBG of the diabetic mice, and increase secretion of the C-peptide、HOMA-IS, that may be show, that the Gln could have effective for diabetes. Model+Gln group compare with model group, there was no significant difference in Bacteroides thetaiotaomicron. that may be show, hypoglycemic effect of Gln was not happened by Bacteroides thetaiotaomicron.