Effect Of Chemical Hypoxia On HepG2Cells And A549Cells

Oxygen serves as an excellent source of life, and plays a key role in life activities,including wound healing, cell respiration, embryonic development and metabolism. In humanbody, different tissues have different oxygen tension. Hypoxia have a variety of damage totissues, hypoxia can result in a large number of physiological and pathophysiologicalprocesses, such as aging process, diabetes, stroke and inflammation cancer.The tumor microenvironment is a very complex system. Evidence indicates that tumorstromal cells play a crucial role in tumor initiation and progression. The tumormicroenvironment is composed of multiple types of tumor stroma cells、extracellular matrixand soluble factors. These interactions modify cellular compartments, leading to theco-evolution of tumor cells and their microenvironment. The microenvironment of mosttumours shows low extracellular pH, low oxygen tension. Hypoxic conditions induce amolecular response that drives the activation of a key transcription factor-HIF-1, It induces atranscription programme that promotes an aggressive tumour phenotype. Tumour hypoxia isassociated with poor prognosis and an increase in the resistance to radio-and chemo-therapy.Target the microenvironment of tumours has thus become a central issue in tumor physiologyand cancer treatment.There are two types of hypoxia in In vitro: physical method and chemical hypoxia.Conventional physical method needs lardge instrumentation, such as nitrogen cylinders. Forthe chemical hypoxia models: cobalt chloride, sodium hyposulfite and sodium azide areuaually used. cobalt is a heavy metal that may lead to cellular toxicity. Another chemicalhypoxia reagent is sodium hyposulfite, the mechanism by which sodium hyposulfite candeprive aqueous solutions of oxygen. This study presented a new type of low toxic chemicaloxygen reagent (sodium sulfite)(Na2SO3) to mimics the tumor hypoxic microenvironment,studying the effect of hypoxia on tumor cells. First we measure the oxygen deprivation effectand hypoxia maintenance ability of sodium sulfite solutions, and we examined the effect ofsodium sulfate(Na2SO4) on HepG2and A549cells. Results shows that Sodium sulfite is apotential hypoxia inducer that mimics hypoxic stress in solid tumor.We systematically studied hypoxia on tumor cell proliferation, cell area, cell perimeter, cell aspect ratio and cytoskeleton.We have designed an effective method that mimics the hypoxia microenvironment that willenable the development of more effective cancer therapies.