Impact Of Pemetrexed On Protein Expression Of SKP2in Lung Adenocarcinoma A549Cells

Objective：To explore the influence of s-phase kinase associated protein2(SKP2)expression and its significance in lung adenocarcinoma A549cells after the treatmentof Pemetrexed(PMX).Methods：According to the concentration of PMX, we set one control group andfour experimental groups:0μM(group A),0.01μM(group B),0.1μM(group C),1μM(group D),10μM(group E). The growth of A549cells handled by differentconcentration PMX for24h,48h,72h,was tested by MTT assay; The effect ofdifferent concentration PMX handling for48h on the cell cycle phase distribution ofA549cells was analyzed by flow cytometry; The expression of SKP2protein of A549cells handled by different concentration PMX for48h was detected by Western blot.Results：MTT assay showed that PMX could significantly inhibit the growth ofA549cells, and the highest inhibition ratio was (78.61±3.66)%at the concentrationof10μM when A549cells were cultured for72h (P<0.01). Meanwhile, s-phase cellsof group C, D, E were more than group A(P<0.05,P＜0.01,P<0.01), and the numberof s-phase cells was increasing with the concertration of PMX. The difference ofSKP2protein expression among each group was significantly(P<0.01), andcompared with control group SKP2protein expression significantly increased inexperimental groups(P<0.01). That increasing was obvious with the increasing ofPMX in certain concentration range.Conclusion：PMX could inhibit the growth of A549cells and arrested them ins-phase. The expression of SKP2protein in PMX-treated A549cells was upregulated,which might be concerned with the chemotherapy effect of PMX.