The Effects Of Chlorogeic Acid On System Of Antioxidant And Amino Acid Metabolism In Intestinal Oxidant Damage Rats

Oxidative damage of the digestive tract is one of the reasons that lead to higher rate of animals diseases. Lipid membrane, metabolic enzymes, functional protein are damaged by oxidative stress in animals intestinal, so that it can not work properly, resulting in metabolic disorders, affecting food digestion and absorption. Therefore, oxidative stress can induce a series of animal syndrome, such as chronic colitis, ulcers, intestine’s peristalsis irregular, metabolic disorders. If it occurs in animals, often it is hard to find and unable to get good treatment, resulting in a number of sicker, eventually leading to serious economic losses. First of all, by acetic acid-induced SD rat colitis model to study the impact of oxidative stress on the chlorogenic acid metabolism. Secondly, the effect of chlorogenic acid of the intestine’s phenolic acids, antioxidant enzyme capacity and GSH content. The last, oxidative stress and chlorogenic acid are effect on the content of AA and Gln enzyme activity. The mechanisms are illustrated that chlorogenic acid can be uesed to repair intestinal oxidative damage.The first part, chlorogenic acid metabolism of the model group is fast than the normal group’s in the jejunum to the colon, whereas no significant difference in the duodenum. The rate of chlorogenic acid metabolism is larger from the duodenum to the colon in turn, the colon is the main site of chlorogenic acid metabolism.The second part, the antioxidant capacity of phenolic acids in the duodenum incubation medium is changed little. From the jejunum to the colon with the incubation time increased, the antioxidant capacity of the model group is higher than the normal group’s. Colon’s antioxidant activity is higher than other bowel; same bowel different incubation times of the same group found that phenolic metabolites generated significantly enhanced antioxidant capacity. Incubated for12h,24h, model group and normal group CAT, SOD, GSH-Px, GST activity have been improved to varying degrees, in which the model group is more significant, a slight increase in GSH content. The third part, Intestinal damage, Glu, Gln, Ala,Ile, His demand increases.Glu synthesis of GSH can effectively scavenge free radicals.His also be converted to Glu.Ala, Ile can be converted to pyruvate to form acetyl coenzyme A into the citric acid cycle to provide energy for the repair of damage.Low doses of CGA barrier repair.The involvement of chlorogenic acid can quickly promote the Gln decomposition converted to Glu, resulting in Gln content decreased, the increase in Glu content, promote the synthesis of GSH, enhanced antioxidant levels.While improving Arg metabolism, promoting the growth and differentiation of intestinal cells, to provide a substrate for protein synthesis, and can also play a clear role of free radical nitric oxide, also involved in the synthesis of GSH may be converted to Glu.Tyr fumaric acid can be formed into the citric acid cycle to provide energy for the repair of damage molecules.Ile, Leu can be converted to pyruvate to form acetyl coenzyme A into the citric acid cycle, provide energy for damage repair.Therefore, Intestinal oxidative stress, low doses of CGA which can regulate intestinal overall antioxidant levels. It also regulates the activity of intestinal Gln and AA fast conversion and utilization, to mitigate and repair the intestinal damage.