Expression Of TNF-α、IL-1β And Influences Of Ulinastatin In Retinal Photic Injury

Objective：Directed by manufacturing idea retinal ultra-violet photic injurymodel in Sprague-Dawley(SD)rat to observe the regularity of expressive changes ofrat retinal tumor necrosis factor α (TNF-α)and interleukin1β(IL-1β)and to explore themechanism and the way of the protection in the retinal photic injury after theinfluence of ulinastatin.Methods：Thirty female SD rats were randomly divided into three groups: thenormal control group、the illuminable control group(ultra-violet exposure) and theexperimental control group(ultra-violet exposure and ulinastatin).The later two werefurther randomly divided into two groups:3d and7d after light exposure.There were6rats (12eyes) in each group. All rats were raised in cyclic environment for sevendays(light environment and dark environment both for12h),then given darkadaptation36h before the experiment.The eyes were enucleated for the normalcontrol group without any interference,and the eyes of both in the illuminable controlgroup and in the experimental control group were given light exposure(caused byultra-violet light)of8h (from8:00Am to5:00Pm)at2200±138Lux.Beginning the lightexposure before three days, ulinastatin（20000u/kg）were administrated to rats inexperimental control group by intraperitoneal injection at8:30Am，for once aday.The all eye balls were enucleated as arranged and made hematoxylin and eosinstaining （HE）to observ the morphological changes of the retina in the lightmicroscope and the TNF-α and IL-1β immunohistochemistry staining to analyze theexpressive changes of TNF-α、IL-1β.The outer nuclear layer （ONL）thinkness andthe expression changes of TNF-α and IL-1β were analyzed and measured usingImage-ProPlus. The data were statistically analyzed by using SPSS19.0for Windows.Results：1.The ideal retinal ultra-violet photic injury model in SD rat were madesuccessfully;2. In the normal control group, there was no morphological changes and expression of TNF-αand IL-1β;3.The retina of the illuminable control groupwas pathologically damaged after3d and7d light exposure,the retina seemed muchmore damaged in the illuminable control group compared to the normal control groupby the measurement of ONL thickness and the expression of TNF-αand IL-1β, and thechanges was statistically different (P<0.01);4. The retina of the experimental controlgroup was pathologically damaged after3d and7d light exposure,and the retinaseemed much less damaged in the experimental control group compared to theilluminable control group by the measurement of ONL thickness and the expressionof TNF-αand IL-1β,but more damaged compared to the normal control group,and thechanges were statistically different (P<0.05).Conclusion:1.The ultra-violet light can cause the retinal photic injury,appearing the damage of the photoreceptors and the apoptosis of visual cells;2.Theexpression of the retinal TNF-α and IL-1β increased after light exposure, then causedthe retinal photic injury and may aggravate the retinal damage;3. Ulinastatin cangive protection to the retinal photic injury by inhibiting the activation of the cells thatcould produce TNF-α and IL-1β and then reduced the oversecretion of TNF-α andIL-1β.